Gomesin acts in the immune system and promotes myeloid differentiation and monocyte/macrophage activation in mouse

Gomesin acts in the immune system and promotes myeloid differentiation and monocyte/macrophage activation in mouse

Author Buri, Marcus Vinicius Autor UNIFESP Google Scholar
Dias, Carolina Carvalho Autor UNIFESP Google Scholar
Barbosa, Christiano Marcello Vaz Autor UNIFESP Google Scholar
Nogueira-Pedro, Amanda Autor UNIFESP Google Scholar
Ribeiro-Filho, Antonio C. Google Scholar
Miranda, Antonio Autor UNIFESP Google Scholar
Paredes-Gamero, Edgar Julian Autor UNIFESP Google Scholar
Abstract Due to the cytotoxic effect of antimicrobial peptides (AMP) against several microorganism and tumor cells has been proposed their association with the immune system. However, just a few reports have shown this relationship. In this study, mice were treated with gomesin, a beta-hairpin AMP that exhibit high cytotoxicity against bacterial and tumor cells. Different effects in the immune system were observed, such as, decrease of CD3(+) in T lymphocytes (Control: 17.7 +/- 1.4%

Gomesin: 7.67 +/- 11.2%) and in hematopoietic progenitors and increase of hematopoietic stem cell (Control: 0.046 +/- 0.004%

Gomesin: 0.067 +/- 10.003%), B220(+) B lymphocytes (Control: 38.63 +/- 1.5%

Gomesin: 47.83 +/- 0.48%), and Mac-1(+)F4/80(+) macrophages (Control: 11.76 +/- 3.4%

Gomesin: 27.13 +/- 4.0%). Additionally, macrophage increase was accompanied by an increase of macrophage phagocytosis (Control 20.85 +/- 1.53

Gomesin 31.32 +/- 1 Geometric mean), interleukin 6 (Control: 47.24 +/- 1.9 ng/mL

Gomesin: 138.68 +/- 33.68 ng/mL) and monocyte chemoattractant protein-1 (Control: 0.872 +/- 0.093 ng/mL

Gomesin: 1.83 +/- 0.067 ng/mL). Thus, this report showed immunomodulatory activity of gomesin in the immune system of mice. (C) 2016 Elsevier Inc. All rights reserved.
Keywords Antimicrobial peptide
Gomesin
Immunomodulatory activity
Macrophage activation
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Sponsor INFAR/UNIFESP Confocal and Flow Cytometry Facility
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2013/09068-7
FAPESP: 2011/17584-0
CNPq: 473797/2013-5
FAPESP: 2012/20989-4
FAPESP: 2015/22901-5
Date 2016
Published in Peptides. New York, v. 85, p. 41-45, 2016.
ISSN 0196-9781 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Inc
Extent 41-45
Origin http://dx.doi.org/10.1016/j.peptides.2016.09.003
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000387299200004
URI https://repositorio.unifesp.br/handle/11600/56854

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