Effects of different physical exercise programs on susceptibility to pilocarpine-induced seizures in female rats

Effects of different physical exercise programs on susceptibility to pilocarpine-induced seizures in female rats

Author Campos, Diego Vannucci Autor UNIFESP Google Scholar
Lopim, Glauber Menezes Autor UNIFESP Google Scholar
Almeida, Vanessa Santos de Autor UNIFESP Google Scholar
Amado, Debora Autor UNIFESP Google Scholar
Arida, Ricardo Mario Autor UNIFESP Google Scholar
Abstract In epilepsy, the most common serious neurological disorder worldwide, several investigations in both humans and animals have shown the effectiveness of physical exercise programs as a complementary therapy. Among the benefits demonstrated, regular exercise can decrease the number of seizures as well as improve cardiovascular and psychological health in people with epilepsy. While many studies in animals have been performed to show the beneficial effects of exercise, they exclusively used male animals. However, females are also worthy of investigation because of their cyclical hormonal fluctuations and possible pregnancy. Considering the few animal studies concerning seizure susceptibility and exercise programs in females, this study aimed to verify whether exercise programs can interfere with seizure susceptibility induced by pilocarpine in adult female Wistar rats. Animals were randomly divided into three groups: control, forced, and voluntary (animals kept in a cage with a wheel). After the final exercise session, animals received a pilocarpine hydrochloride (350 mg/kg i.p.

Sigma) injection to induce seizures. To measure the intensity of pilocarpine-induced motor signs, we used a scale similar to that developed by Racine (1972) in the kindling model. During a 4-h period of observation, we recorded latency for first motor signs, latency for reaching SE, number of animals that developed SE, and intensity of pilocarpine-induced motor signs. No difference was observed among groups in latency for first motor signs and in the number of animals that developed SE. Although the voluntary group presented more intense motor signs, an increased latency for developing SE was observed compared with that in forced and control groups. Our behavioral results are not enough to explain physiological and molecular pathways, but there are mechanisms described in literature which may allow us to propose possible explanations. Voluntary exercise increased latency to SE development. Further investigation is necessary to elucidate the pathways involved in these results, while more studies should be performed regarding gender specific differences. (C) 2016 Elsevier Inc. All rights reserved.
Keywords Epilepsy
Seizures susceptibility
Female rats
xmlui.dri2xhtml.METS-1.0.item-coverage San Diego
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2015/19256-0
CNPq: 300605/2013-0
Date 2016
Published in Epilepsy & Behavior. San Diego, v. 64, p. 262-267, 2016.
ISSN 1525-5050 (Sherpa/Romeo, impact factor)
Publisher Academic Press Inc Elsevier Science
Extent 262-267
Origin http://dx.doi.org/10.1016/j.yebeh.2016.08.011
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000389734800045
URI https://repositorio.unifesp.br/handle/11600/56790

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