Microfluidic generation of alginate microgels for the controlled delivery of lentivectors

Microfluidic generation of alginate microgels for the controlled delivery of lentivectors

Author Madrigal, Justin L. Google Scholar
Stilhano, Roberta S. Google Scholar
Siltanen, Christian Google Scholar
Tanaka, Kimberly Google Scholar
Rezvani, Sabah N. Google Scholar
Morgan, Ryan P. Google Scholar
Revzin, Alexander Google Scholar
Han, Sang Won Autor UNIFESP Google Scholar
Silva, Eduardo A. Google Scholar
Abstract Lentivectors are widely used for gene delivery and have been increasingly tested in clinical trials. However, achieving safe, localized, and sufficient gene expression remain key challenges for effective lentivectoral therapy. Localized and efficient gene expression can be promoted by developing material systems to deliver lentivectors. Here, we address the utility of microgel encapsulation as a strategy for the controlled release of lentivectors. Three distinct routes for ionotropic gelation of alginate were incorporated into microfluidic templating to create lentivector-loaded microgels. Comparisons of the three microgels revealed marked differences in mechanical properties, crosslinking environment, and ultimately lentivector release and functional gene expression in vitro. Gelation with chelated calcium demonstrated low utility for gene delivery due to a loss of lentivector function with acidic gelation conditions. Both calcium carbonate gelation, and calcium chloride gelation, preserved lentivector function with a more sustained transduction and gene expression over 4 days observed with calcium chloride gelated microgels. The validation of these two strategies for lentivector microencapsulation may provide a platform for controlled gene delivery.
xmlui.dri2xhtml.METS-1.0.item-coverage Cambridge
Language English
Sponsor American Heart Association
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Hellman Family
Howard Hughes Medical Institute (HHMI) Integrating Medicine into Basic Science
University of California, Davis
Grant number American Heart Association: 15BGIA25730057
FAPESP: 2015/20206-8
FAPESP: 2012/00753-6
Date 2016
Published in Journal Of Materials Chemistry B. Cambridge, v. 4, n. 43, p. 6989-6999, 2016.
ISSN 2050-750X (Sherpa/Romeo, impact factor)
Publisher Royal Soc Chemistry
Extent 6989-6999
Origin http://dx.doi.org/10.1039/c6tb02150f
Access rights Closed access
Type Article
Web of Science ID WOS:000387882000008
URI https://repositorio.unifesp.br/handle/11600/56707

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