Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Author Braga, Daniela Paes Almeida Ferreira Autor UNIFESP Google Scholar
Setti, Amanda Souza Google Scholar
Lo Turco, Edson Guimaraes Autor UNIFESP Google Scholar
Cordeiro, Fernanda Bertuccez Autor UNIFESP Google Scholar
Cabral, Elaine Cristina Google Scholar
Cortezzi, Sylvia Sanches Google Scholar
Ono, Erika Google Scholar
Savio Figueira, Rita Cassia Google Scholar
Eberlin, Marcos Nogueira Google Scholar
Borges Júnior, Edson Autor UNIFESP Google Scholar
Abstract The goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs). Forty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negative-pregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database. There were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the non-blastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group. CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.
Keywords Cumulus cell
Blastocyst
Mass spectrometry
Pregnancy
Proteomics
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2013/50052-7
Date 2016
Published in Journal Of Assisted Reproduction And Genetics. New York, v. 33, n. 12, p. 1571-1583, 2016.
ISSN 1058-0468 (Sherpa/Romeo, impact factor)
Publisher Springer/Plenum Publishers
Extent 1571-1583
Origin http://dx.doi.org/10.1007/s10815-016-0800-7
Access rights Closed access
Type Article
Web of Science ID WOS:000390025000006
URI https://repositorio.unifesp.br/handle/11600/56636

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