Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine

Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine

Author Eboli, Ligia Patricia de Carvalho Batista Autor UNIFESP Google Scholar
Salzedas-Netto, Alcides Augusto Autor UNIFESP Google Scholar
Azevedo, Ramiro Antero de Autor UNIFESP Google Scholar
Lanzoni, Valeria Pereira Autor UNIFESP Google Scholar
Paula, Tatiana Sugayama de Autor UNIFESP Google Scholar
Goldenberg, Alberto Autor UNIFESP Google Scholar
Gonzalez, Adriano Miziara Autor UNIFESP Google Scholar
Abstract PURPOSE: To describe an animal model for acute liver failure by intraperitoneal d-galactosamine injections in rats and to define when is the best time to intervene through King's College and Clichy's criteria evaluation. METHODS: Sixty-one Wistar female rats were distributed into three groups: group 1 (11 rats received 1.4 g/kg of d-galactosamine intraperitoneally and were observed until they died)

group 2 (44 rats received a dose of 1.4 g/kg of d-galactosamine and blood and histological samples were collected for analysis at 12, 24, 48, 72 and 120 hours after the injection)

and the control group as well (6 rats). RESULTS: Twelve hours after applying d-galactosamine, AST/ALT, bilirubin, factor V, PT and INR were already altered. The peak was reached at 48 hours. INR > 6.5 was found 12 hours after the injection and factor V < 30% after 24 hours. All the laboratory variables presented statistical differences, except urea (p = 0.758). There were statistical differences among all the histological variables analyzed. CONCLUSION: King's College and Clichy's criteria were fulfilled 12 hours after the d-galactosamine injection and this time may represent the best time to intervene in this acute liver failure animal model.
Keywords Liver Failure, Acute
Galactosamine
Models, Animal
Rats
xmlui.dri2xhtml.METS-1.0.item-coverage Sao Paulo
Language English
Date 2016
Published in Acta Cirurgica Brasileira. Sao Paulo, v. 31, n. 12, p. 783-792, 2016.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Extent 783-792
Origin http://dx.doi.org/10.1590/S0102-865020160120000002
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000393790500002
SciELO ID S0102-86502016001200783 (statistics in SciELO)
URI https://repositorio.unifesp.br/handle/11600/56577

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