Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice

Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice

Author Bella, Leonardo M. Google Scholar
Fieri, Isis Google Scholar
Tessaro, Fernando H. G. Google Scholar
Nolasco, Eduardo L. Google Scholar
Nunes, Fernanda P. B. Google Scholar
Ferreira, Sabrina S. Google Scholar
Azevedo, Carolina B. Autor UNIFESP Google Scholar
Martins, Joilson O. Google Scholar
Abstract Background/Aims. The effects of cholecalciferol supplementation on the course of diabetes in humans and animals need to be better understood. Therefore, this study investigated the effect of short-term cholecalciferol supplementation on biochemical and hematological parameters in mice. Methods. Male diabetic (alloxan, 60mg/kg i.v., 10 days) and non diabetic mice were supplemented with cholecalciferol for seven days. The following parameters were determined: serum levels of 25-hydroxyvitamin D, phosphorus, calcium, urea, creatinine, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, red blood cell count, white blood cell count (WBC), hematocrit, hemoglobin, differential cell counts of peritoneal lavage (PeL), and bronchoalveolar lavage (BAL) fluids and morphological analysis of lung, kidney, and liver tissues. Results. Relative to controls, cholecalciferol supplementation increased serum levels of 25-hydroxyvitamin D, calcium, hemoglobin, hematocrit, and red blood cell counts and decreased leukocyte cell counts of PeL and BAL fluids in diabetic mice. Diabetic mice that were not treated with cholecalciferol had lower serum calcium and albumin levels and hemoglobin, WBC, and mononuclear blood cell counts and higher serum creatinine and urea levels than controls. Conclusion. Our results suggest that cholecalciferol supplementation improves the hematological parameters and reduces leukocyte migration into the PeL and BAL lavage of diabetic mice.
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Pro-reitoria de Pesquisa da Universidade de Sao Paulo (PRP/USP, Projeto I and Novos Docentes)
Grant number FAPESP: 2010/02272-0
FAPESP: 2012/23998-4
FAPESP: 2013/20904-1
FAPESP: 2014/05214-1
FAPESP: 2017/05100-4
CNPq: 470523/2013-1
CNPq: 301617/2016-3
Date 2017
Published in Biomed Research International. London, v. , p. -, 2017.
ISSN 2314-6133 (Sherpa/Romeo, impact factor)
Publisher Hindawi Ltd
Extent -
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000399972200001

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