Acute crack cocaine exposure induces genetic damage in multiple organs of rats

Acute crack cocaine exposure induces genetic damage in multiple organs of rats

Author Moretti, Eduardo Gregolin Autor UNIFESP Google Scholar
Yujra, Veronica Quispe Autor UNIFESP Google Scholar
Claudio, Samuel Rangel Autor UNIFESP Google Scholar
Silva, Marcelo Jose Dias Google Scholar
Vilegas, Wagner Google Scholar
Pereira, Camilo Dias Seabra Autor UNIFESP Google Scholar
Oliveira, Flavia de Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Abstract Crack cocaine is a very toxic product derived from cocaine. The aim of this study was to evaluate genetic damage in multiple organs of rats following acute exposure to crack cocaine. A total of 20 Wistar rats were distributed into four groups (n = 5), as follows: 0, 4.5, 9, and 18 mg/kg body weight (b.w.) of crack cocaine administered by intraperitoneal route (i.p.). All animals were killed 24 h after intraperitoneal (i.p.) injection. The results showed that crack cocaine increased the number of micronucleated cells in bone marrow cells exposed to 18 mg/kg crack cocaine (p < 0.05). Peripheral blood and liver cells presented genetic damage as depicted by single cell gel (comet) assay at 9 and 18 mg/kg doses (p < 0.05). Immunohistochemistry data revealed significant increase in 8-hydroxy-20-deoxyguanosine (8-OHdG) immunoexpression in hepatocytes of animals exposed to crack cocaine at 9 and 18 mg/kg (p < 0.05) when compared with negative controls. Taken together, our results demonstrate that crack cocaine is able to induce genomic damage in multiple organs of Wistar rats.
Keywords Crack cocaine
Genomic instability
DNA damage
Rat
xmlui.dri2xhtml.METS-1.0.item-coverage Heidelberg
Language English
Sponsor Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Coordenacao de Aperfeicoamento de Pessoal de NIvel Superior (CAPES)
Date 2016
Published in Environmental Science And Pollution Research. Heidelberg, v. 23, n. 8, p. 8104-8112, 2016.
ISSN 0944-1344 (Sherpa/Romeo, impact factor)
Publisher Springer Heidelberg
Extent 8104-8112
Origin http://dx.doi.org/10.1007/s11356-016-6141-3
Access rights Closed access
Type Article
Web of Science ID WOS:000374994600100
URI https://repositorio.unifesp.br/handle/11600/56111

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