O complexo destruidor de betacatenina no carcinoma colorretal e no adenoma cólico

O complexo destruidor de betacatenina no carcinoma colorretal e no adenoma cólico

Alternative title The destruction complex of beta-catenin in colorectal carcinoma and colonic adenoma
Author Bourroul, Guilherme Muniz Google Scholar
Fragoso, Helio Jose Google Scholar
Gomes, Jose Walter Feitosa Google Scholar
Bourroul, Vivian Sati Oba Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Gomes, Thiago Simao Autor UNIFESP Google Scholar
Saba, Gabriela Tognini Google Scholar
Palma, Rogerio Tadeu Google Scholar
Waisberg, Jaques Autor UNIFESP Google Scholar
Abstract Objective: To evaluate the destruction complex of beta-catenin by the expression of the proteins beta-catetenin, adenomatous polyposis coli, GSK3 beta, axin and ubiquitin in colorectal carcinoma and colonic adenoma. Methods: Tissue samples from 64 patients with colorectal carcinoma and 53 patients with colonic adenoma were analyzed. Tissue microarray blocks and slides were prepared and subjected to immunohistochemistry with polyclonal antibodies in carcinoma, adjacent non-neoplastic mucosa, and adenoma tissues. The immunoreactivity was evaluated by the percentage of positive stained cells and by the intensity assessed through of the stained grade of proteins in the cytoplasm and nucleus of cells. In the statistical analysis, the Spearman correlation coefficient, Student's t, chi(2), Mann-Whitney, and McNemar tests, and univariate logistic regression analysis were used. Results: In colorectal carcinoma, the expressions of beta-catenin and adenomatous polyposis coli proteins were significantly higher than in colonic adenomas (p<0.001 and p<0.0001, respectively). The immunoreactivity of GSK3 beta, axin 1 and ubiquitin proteins was significantly higher (p=0.03, p=0.039 and p=0.03, respectively) in colorectal carcinoma than in the colonic adenoma and adjacent non-neoplastic mucosa. The immunohistochemistry staining of these proteins did not show significant differences with the clinical and pathological characteristics of colorectal cancer and colonic adenoma. Conclusions: These results suggest that, in adenomas, the lower expression of the beta-catenin, axin 1 and GSK3 beta proteins indicated that the destruction complex of beta-catenin was maintained, while in colorectal carcinoma, the increased expression of beta-catenin, GSK3 beta, axin 1, and ubiquitin proteins indicated that the destruction complex of beta-catenin was disrupted.

Objetivo: Avaliar o complexo de destruição da betacatenina no carcinoma colorretal e no adenoma do colo pela expressão das proteínas betacatenina, adenomatous polyposis coli, GSK3β, axina e ubiquitina. Métodos: Amostras de tecidos de 64 doentes com carcinoma colorretal e de 53 pacientes com adenoma do colo foram analisadas. Blocos de tecidos foram submetidos ao estudo imuno-histoquímico com anticorpos policlonais nos tecidos do carcinoma, mucosa não neoplásica adjacente e adenoma. A imunorreatividade foi avaliada pela porcentagem de positividade de células coradas e pela intensidade do grau de coloração das proteínas no citoplasma e no núcleo das células. Na análise estatística, foram utilizados o coeficiente de correlação de Spearman, os testes t de Student, χ2, Mann-Whitney e de McNemar, e a análise de regressão logística univariada. Resultados: No carcinoma colorretal, as expressões da betacatenina e da adenomatous polyposis coli foram significativamente maiores do que em adenomas do colo (p<0,001 e p<0,0001, respectivamente). A imunorreatividade das proteínas GSK3β, axina 1 e ubiquitina foi significativamente maior (p=0,03, p=0,039 e p=0,03, respectivamente) no carcinoma colorretal do que no adenoma e na mucosa não neoplásica adjacente. A coloração imuno-histoquímica dessas proteínas não apresentou diferenças significantes em relação às características clinicopatológicas do câncer colorretal e do adenoma. Conclusões: Em adenomas, as menores expressões de betacatenina, axina 1 e GSK3β indicaram que o complexo de destruição da betacatenina estava conservado, enquanto que, no carcinoma colorretal, o aumento das expressões da betacatenina, GSK3β, 1 axina, e ubiquitina indicaram que o complexo de destruição de betacatenina estava alterado.
Keywords Colorectal neoplasms
Adenoma
Immunohistochemistry
beta Catenin
Genes, APC
Glycogen synthase
Axin protein
Ubiquitin
Wnt signaling pathway
Neoplasias colorretais
Adenoma
Imuno-histoquímica
beta Catenina
Genes APC
Glicogênio sintase
Proteína axina
Ubiquitina
Via de sinalização Wnt
xmlui.dri2xhtml.METS-1.0.item-coverage Sao Paulo Sp
Language English
Portuguese
Date 2016
Published in Einstein-Sao Paulo. Sao Paulo Sp, v. 14, n. 2, p. 135-142, 2016.
ISSN 1679-4508 (Sherpa/Romeo, impact factor)
Publisher Inst Israelita Ensino & Pesquisa Albert Einstein
Extent 135-142
Origin http://dx.doi.org/10.1590/S1679-45082016AO3678
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000385717300006
SciELO ID S1679-45082016000200006 (statistics in SciELO)
URI https://repositorio.unifesp.br/handle/11600/56067

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