Central and peripheral effects of physical exercise without weight reduction in obese and lean mice

Central and peripheral effects of physical exercise without weight reduction in obese and lean mice

Author Carvalho, Francine Pereira de Autor UNIFESP Google Scholar
Moretto, Thais Ludmilla Autor UNIFESP Google Scholar
Benfato, Izabelle Dias Autor UNIFESP Google Scholar
Barthichoto, Marcela Autor UNIFESP Google Scholar
Ferreira, Sandra Mara Google Scholar
Costa-Junior, Jose Maria Google Scholar
Oliveira, Camila Aparecida Machado de Autor UNIFESP Google Scholar
Abstract To investigate the central (hypothalamic) and peripheral effects of exercise without body weight change in diet-induced obesity (DIO). Twelve-week-old male C57Bl/6 mice received a control (C) or a high-fat diet (H). Half of them had free access to running wheels for 5 days/week for 10 weeks (CE) and HE, respectively). Hypothalamic expression of genes related to energy homeostasis, and leptin (Stat3 and p-Stat3) and insulin (Akt and p-Akt) signaling were evaluated. Glucose and leptin tolerance, peripheral insulin sensitivity, and plasma insulin, leptin and adiponectin were determined. Perigonadal and retroperitoneal fat depots were increased by diet but reduced by exercise despite lack of effect of exercise on body weight. Blood glucose during intraperitoneal glucose tolerance test (ipGTT) was higher and glucose decay during intraperitoneal insulin tolerance test (ipITT) was lower in H and HE compared with C and CE. Exercise increased liver p-Akt expression and reduced fast glycemia. High-fat diet increased plasma insulin and leptin. Exercise had no effect on insulin but decreased leptin and increased adiponectin. Leptin inhibited food intake in all groups. Hypothalamic total and p-Stat3 and Akt were similar amongst the groups despite higher plasma levels of leptin and insulin in H and HE mice. High-fat diet modulated gene expression favoring a positive energy balance. Exercise only marginally changed the gene expression. Exercise induced positive changes (decreased fast glycemia and fat depots; increased liver insulin signaling and adiponectin concentration) without weight loss. Thus, despite reducing body weight could bring additional benefits, the effects of exercise must not be overlooked when weight reduction is not achieved.
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2011/05932-3
FAPESP: 2013/01624-8
Date 2018
Published in Bioscience Reports. London, v. 38, p. -, 2018.
ISSN 0144-8463 (Sherpa/Romeo, impact factor)
Publisher Portland Press Ltd
Extent -
Origin http://dx.doi.org/10.1042/BSR20171033
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000431504200007
URI https://repositorio.unifesp.br/handle/11600/55624

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