Synthesis and characterization of aminolevulinic acid gold nanoparticles: Photo and sonosensitizer agent for atherosclerosis

Synthesis and characterization of aminolevulinic acid gold nanoparticles: Photo and sonosensitizer agent for atherosclerosis

Author Goncalves, Karina de Oliveira Autor UNIFESP Google Scholar
Vieira, Daniel Perez Autor UNIFESP Google Scholar
Courrol, Lilia Coronato Autor UNIFESP Google Scholar
Abstract Photodynamic and sonodynamic therapies (PDT and SDT, respectively) are emerging as new atherosclerosis treatments. The subsequent generation of free radicals by activated photo and sonosensitizers can lead to apoptotic cell death. The use of gold nanoparticles (AuNPs) as the vehicle for a sensitizer delivery improves reactive oxygen species formation and sensitizer performance. In this study gold nanoparticles functionalized with polyethylene glycol (PEG) were synthesized mixing delta-aminolevulinic acid (ALA) with tetrachloroauric (III) acid in milliQ water solution followed by photo reduction with 300W xenon lamp. The synthesized ALA: AuNPs were characterized by UV/vis optical absorption, zeta potential and electron microscopy. The mean particle size of spherical ALA: AuNP was similar to 18 nm, with a polydispersity index of 0.437. Singlet oxygen generation efficiency was measured using the trap molecule 1,3-diphenylisobenzofuran. ALA: AuNPs and DPBF were irradiated with 590 nm LED, or pulse ultrasound irradiation (1 W/cm(2) with 1.0 MHz), and consumption of the DPBF was monitored over time by the absorption and emission spectra. The results showed that he gold nanoparticles generate singlet oxygen during light and ultrasound irradiations. THP-1 cells differentiated into macrophages cytotoxicity test were described and was found the half maximal inhibitory concentration (IC50) values similar to 36 nM for ALA: AuNPs. Increase in the fluorescence intensity of PpIX extracted from macrophages incubated with ALA: AuNPs indicating stable encapsulation of ALA into gold nanoparticles and further conversion to PpIX. The potential use of ALA: AuNps as a sensitizer for photo and sonodynamic therapies were investigated. ALA: AuNPs mediated SDT was more effective than PDT. SDT with ALA: AuNPs induced the reduction of macrophage viability in similar to 87,5% in only 2 min. The mechanism underlying SDT-induced apoptosis involves the generation of singlet oxygen. The results indicate that ALA: AuNPs can be used as a novel photo and sonosensitizer for atherosclerosis.
Keywords PDT
SDT
Atherosclerosis
ALA
Gold nanoparticles
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Grant number FAPESP: 2010/016544-1
Date 2018
Published in Journal Of Luminescence. Amsterdam, v. 197, p. 317-323, 2018.
ISSN 0022-2313 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 317-323
Origin http://dx.doi.org/10.1016/j.jlumin.2018.01.057
Access rights Closed access
Type Article
Web of Science ID WOS:000425996400046
URI https://repositorio.unifesp.br/handle/11600/55621

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