Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress

Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress

Author Adami, L. N. G. Autor UNIFESP Google Scholar
Belardin, Larissa Berloffa Autor UNIFESP Google Scholar
Lima, B. T. Autor UNIFESP Google Scholar
Jeremias, J. T. Autor UNIFESP Google Scholar
Antoniassi, Mariana Pereira Autor UNIFESP Google Scholar
Okada, Fatima Kazue Autor UNIFESP Google Scholar
Bertolla, Ricardo Pimenta Autor UNIFESP Google Scholar
Abstract The aim of this study was to evaluate the antioxidant effect of in vitro supplementation with vitamin E in human spermatozoon incubated with an oxidative stress inducer. In this study, semen samples from 30 patients were collected and with one aliquot we performed semen analysis according to WHO. The remaining volume was divided into four aliquots: group C: incubated with BWW medium

group I: incubated with 5mmol 1-1 hydrogen peroxide

group A: incubated with 40mol 1-1 vitamin E

and group AI: incubated with both them. After incubations, sperm functional analyses were performed and included: evaluation of oxidative stress, acrosome integrity, mitochondrial activity and DNA fragmentation. Groups were compared using a Friedman test with Bonferroni post hoc (=5%). In this study, we observed that in group I there was a decrease in acrosome integrity and mitochondrial activity, and an increase in DNA fragmentation, when compared to group C. Group AI showed an increase in acrosome integrity and mitochondrial activity when compared with group I. Based on our findings, we conclude that the vitamin E supplementation had a positive effect in protecting human spermatozoon from induced oxidative stress.
Keywords antioxidant
male infertility
oxidative stress
reactive oxygen species
vitamin E
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor FAPESP
Grant number FAPESP: 2015/02955-3
CNPq: 306616/2013-0
Date 2018
Published in Andrologia. Hoboken, v. 50, n. 4, p. -, 2018.
ISSN 0303-4569 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent -
Origin http://dx.doi.org/10.1111/and.12959
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000429536900018
URI https://repositorio.unifesp.br/handle/11600/55602

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