Reflection imaging of China ink-perfused brain vasculature using confocal laser-scanning microscopy after clarification of brain tissue by the Spalteholz method

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dc.contributor.author Gutierre, R. C. [UNIFESP]
dc.contributor.author Vannucci Campos, D. [UNIFESP
dc.contributor.author Mortara, R. A. [UNIFESP
dc.contributor.author Coppi, A. A.
dc.contributor.author Arida, R. M. [UNIFESP
dc.date.accessioned 2020-07-17T14:02:34Z
dc.date.available 2020-07-17T14:02:34Z
dc.date.issued 2017
dc.identifier http://dx.doi.org/10.1111/joa.12578
dc.identifier.citation Journal Of Anatomy. Hoboken, v. 230, n. 4, p. 601-606, 2017.
dc.identifier.issn 0021-8782
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/54867
dc.description.abstract Confocal laser- scanning microscopy is a useful tool for visualizing neurons and glia in transparent preparations of brain tissue from laboratory animals. Currently, imaging capillaries and venules in transparent brain tissues requires the use of fluorescent proteins. Here, we show that vessels can be imaged by confocal laser- scanning microscopy in transparent cortical, hippocampal and cerebellar preparations after clarification of China inkinjected specimens by the Spalteholz method. This method may be suitable for global, three- dimensional, quantitative analyses of vessels, including stereological estimations of total volume and length and of surface area of vessels, which constitute indirect approaches to investigate angiogenesis. en
dc.description.sponsorship CAPES PNPD
dc.description.sponsorship FAPESP
dc.description.sponsorship CNPq
dc.description.sponsorship Instituto Nacional de NeurocienciaTranslacional (INNT)
dc.format.extent 601-606
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartof Journal Of Anatomy
dc.rights Acesso aberto
dc.subject brain en
dc.subject clarification en
dc.subject confocal microscopy en
dc.subject stereology en
dc.subject vessels en
dc.title Reflection imaging of China ink-perfused brain vasculature using confocal laser-scanning microscopy after clarification of brain tissue by the Spalteholz method en
dc.type Artigo
dc.description.affiliation Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Neurol & Neurosurg, Sao Paulo, Brazil
dc.description.affiliation Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliation Univ Surrey, Fac Hlth & Med Sci, Sch Vet Med, Guildford, Surrey, England
dc.description.affiliation Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Physiol, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Neurol & Neurosurg, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Physiol, Sao Paulo, Brazil
dc.identifier.doi 10.1111/joa.12578
dc.description.source Web of Science
dc.identifier.wos WOS:000398042300011
dc.coverage Hoboken
dc.citation.volume 230
dc.citation.issue 4



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