Reflection imaging of China ink-perfused brain vasculature using confocal laser-scanning microscopy after clarification of brain tissue by the Spalteholz method

Reflection imaging of China ink-perfused brain vasculature using confocal laser-scanning microscopy after clarification of brain tissue by the Spalteholz method

Author Gutierre, R. C. Autor UNIFESP Google Scholar
Vannucci Campos, D. [UNIFESP Google Scholar
Mortara, R. A. [UNIFESP Google Scholar
Coppi, A. A. Google Scholar
Arida, R. M. [UNIFESP Google Scholar
Abstract Confocal laser- scanning microscopy is a useful tool for visualizing neurons and glia in transparent preparations of brain tissue from laboratory animals. Currently, imaging capillaries and venules in transparent brain tissues requires the use of fluorescent proteins. Here, we show that vessels can be imaged by confocal laser- scanning microscopy in transparent cortical, hippocampal and cerebellar preparations after clarification of China inkinjected specimens by the Spalteholz method. This method may be suitable for global, three- dimensional, quantitative analyses of vessels, including stereological estimations of total volume and length and of surface area of vessels, which constitute indirect approaches to investigate angiogenesis.
Keywords brain
clarification
confocal microscopy
stereology
vessels
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor CAPES PNPD
FAPESP
CNPq
Instituto Nacional de NeurocienciaTranslacional (INNT)
Date 2017
Published in Journal Of Anatomy. Hoboken, v. 230, n. 4, p. 601-606, 2017.
ISSN 0021-8782 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent 601-606
Origin http://dx.doi.org/10.1111/joa.12578
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000398042300011
URI https://repositorio.unifesp.br/handle/11600/54867

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