Characterization of Rabbit Corneas Subjected to Stromal Stiffening by the Acai Extract (Euterpe oleracea)

Characterization of Rabbit Corneas Subjected to Stromal Stiffening by the Acai Extract (Euterpe oleracea)

Author Bersanetti, Patricia A. Autor UNIFESP Google Scholar
Bueno, Tatiane L. N. Autor UNIFESP Google Scholar
Morandim-Giannetti, Andreia de A. Google Scholar
Nogueira, Regina F. Autor UNIFESP Google Scholar
Matos, Jivaldo R. Google Scholar
Schor, Paulo Autor UNIFESP Google Scholar
Abstract Purpose: In this study, we characterized rabbit corneas subjected to corneal cross-linking (CXL) with acai extract compared with a riboflavin photo-stimulated procedure. Materials and Methods: The corneas of the slaughterhouse rabbits were divided into three groups: control, consisting of untreated corneal samples

riboflavin/UVA, where corneas were treated with 0.1% riboflavin photo-stimulated at 365 nm as the standard protocol

and acai, where the samples were subjected to 4% acai extract for 0.5-2 h. After the CXL procedure, corneas of the three groups were characterized by analyzing their elastic modulus and thermal denaturation profile. Results: The elastic modulus at 3% strain showed an approximately threefold increase in the riboflavin/UVA group and 10.5 times in the corneas treated with 4% acai extract for 2 h, compared with the control group (p < 0.01). The denaturation temperature values of the two groups of crosslinked corneas increased significantly (p < 0.05) and were more pronounced in the acai group. Conclusions: The acai extract was effective in promoting CXL in rabbit corneas as characterized by the different techniques.
Keywords Acai extract
corneal crosslinking
differential scanning calorimetry
elastic modulus
keratoconus
riboflavin
xmlui.dri2xhtml.METS-1.0.item-coverage Philadelphia
Language English
Sponsor Sao Paulo Research Foundation (FAPESP)
National Council for Scientific and Technological Development (CNPq)
Grant number FAPESP: 2013/20857-3
FAPESP: 2012/07343-8
Date 2017
Published in Current Eye Research. Philadelphia, v. 42, n. 4, p. 528-533, 2017.
ISSN 0271-3683 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Inc
Extent 528-533
Origin http://dx.doi.org/10.1080/02713683.2016.1214970
Access rights Closed access
Type Article
Web of Science ID WOS:000399609900006
URI https://repositorio.unifesp.br/handle/11600/54821

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