Glycosaminoglycan distribution in the rat uterine cervix during the estrous cycle

Glycosaminoglycan distribution in the rat uterine cervix during the estrous cycle

Author Cubas, Jairo Jose Matozinho Autor UNIFESP Google Scholar
Simões, Ricardo Santos Google Scholar
Oliveira-Filho, Ricardo Martins Google Scholar
Simões, Manuel de Jesus Autor UNIFESP Google Scholar
Baracat, Edmund Chada Autor UNIFESP Google Scholar
Soares-Junior, José Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract OBJECTIVE: To analyze the amount of glycosaminoglycans in the uterine cervix during each phase of the rat estrous cycle. DESIGN: Based on vaginal smears, forty female, regularly cycling rats were divided into four groups (n = 10 for each group): GI - proestrous, GII - estrous, GIII - metaestrous and GIV - diestrous. Animals were sacrificed at each phase of the cycle, and the cervix was immediately removed and submitted to biochemical extraction and determination of sulfated glycosaminoglycans and hyaluronic acid. The results were analyzed by ANOVA followed by the Bonferroni post-hoc test. RESULTS: The uterine cervix had the highest amount of total sulfated glycosaminoglycans and dermatan sulfate during the estrous phase (8.90 ± 0.55 mg/g of cetonic extract, p<0.001; and 8.86 ± 0.57 mg/g of cetonic extract, p<0.001). In addition, there was more heparan sulfate at the cervix during the proestrous phase (0.185 ± 0.03 mg/g of cetonic extract) than during any other phase (p<0.001). There were no significant changes in the concentration of hyaluronic acid in the uterine cervix during the estrous cycle. CONCLUSION: Our data suggest that the amount of total sulfated glycosaminoglycans may be influenced by hormonal fluctuations related to the estrous cycle, with dermatan sulfate and heparan sulfate being the glycosaminoglycans most sensitive to hormonal change.
Keywords Heparan sulfate
Dermatan sulfate
Hyaluronic acid
Cervix uteri
Estrous cycle
Language English
Date 2010-01-01
Published in Clinics. Faculdade de Medicina / USP, v. 65, n. 7, p. 703-708, 2010.
ISSN 1807-5932 (Sherpa/Romeo, impact factor)
Publisher Faculdade de Medicina / USP
Extent 703-708
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000280808400009
SciELO ID S1807-59322010000700009 (statistics in SciELO)

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