Altered MT1 and MT2 melatonin receptors expression in the hippocampus of pilocarpine-induced epileptic rats

Altered MT1 and MT2 melatonin receptors expression in the hippocampus of pilocarpine-induced epileptic rats

Author Alves de Alencar Rocha, Anna Karynna Autor UNIFESP Google Scholar
de Lima, Eliangela Autor UNIFESP Google Scholar
Amaral, Fernanda Autor UNIFESP Google Scholar
Peres, Rafael Google Scholar
Cipolla-Neto, Jose Google Scholar
Amado, Debora Autor UNIFESP Google Scholar
Abstract Clinical and experimental findings show that melatonin may be used as an adjuvant to the treatment of epilepsy related complications by alleviates sleep disturbances, circadian alterations and attenuates seizures alone or in combination with AEDs. In addition, it has been observed that there is a circadian component on seizures, which cause changes in circadian system and in melatonin production. Nevertheless, the dynamic changes of the melatoninergic system, especially with regard to its membrane receptors (MT1 and MT2) in the natural course of TLE remain largely unknown. The aim of this study was to evaluate the 24-hour profile of MT1 and MT2 mRNA and protein expression in the hippocampus of rats submitted to the pilocarpine-induced epilepsy model analyzing the influence of the circadian rhythm in the expression pattern during the acute, silent, and chronic phases. Melatonin receptor MT1 and MT2 mRNA expression levels were increased in the hippocampus of rats few hours after SE, with MI1 returning to normal levels and MT2 reducing during the silent phase. During the chronic phase, mRNA expression levels of both receptors return to levels close to control, however, presenting a different daily profile, showing that there is a circadian change during the chronic phase. Also, during the acute and silent phase it was possible to verify MT1 label only in CA2 hippocampal region with an increased expression only in the dark period of the acute phase. The MT2 receptor was present in all hippocampal regions, however, it was reduced in the acute phase and it was found in astrocytes. In chronic animals, there is a reduction in the presence of both receptors especially in regions where there is a typical damage derived from epilepsy. Therefore, we conclude that SE induced by pilocarpine is able to change melatonin receptor MT1 and MT2 protein and mRNA expression levels in the hippocampus of rats few hours after SE as well as in silent and chronic phases. (C) 2017 Elsevier Inc. All rights reserved.
Keywords Epilepsy
Pilocarpine
Melatonin receptors
xmlui.dri2xhtml.METS-1.0.item-coverage San Diego
Language English
Date 2017
Published in Epilepsy & Behavior. San Diego, v. 71, p. 23-34, 2017.
ISSN 1525-5050 (Sherpa/Romeo, impact factor)
Publisher Academic Press Inc Elsevier Science
Extent 23-34
Origin http://dx.doi.org/10.1016/j.yebeh.2017.01.020
Access rights Closed access
Type Article
Web of Science ID WOS:000404201300005
URI https://repositorio.unifesp.br/handle/11600/54343

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