In vitro cytotoxicity of chemical preservatives on human fibroblast cells

In vitro cytotoxicity of chemical preservatives on human fibroblast cells

Author Spindolau, Daniel Gonsales Autor UNIFESP Google Scholar
Hinsberger, Andre Google Scholar
de Souza Antunes, Valeria Maria Google Scholar
Gomes Michelin, Luis Felipe Google Scholar
Bincoletto, Claudia Autor UNIFESP Google Scholar
Oliveira, Carlos Rocha Autor UNIFESP Google Scholar
Abstract Preservatives are widely used substances that are commonly added to various cosmetic and pharmaceutical products to prevent or inhibit microbial growth. In this study, we compared the in vitro cytotoxicity of different types of currently used preservatives, including methylparaben, imidazolidinyl urea (IMU), and sodium benzoate, using the human newborn fibroblast cell line CCD 1072Sk. Of the tested preservatives, only IMU induced a reduction in cell viability, as shown using the MIT assay and propidium iodide staining (IMU > methylparaben > sodium benzoate). IMU was shown to promote homeostatic alterations potentially related to the initiation of programed cell death, such as decreased mitochondrial membrane potential and caspase-3 activation, in the treated cells Methylparaben and sodium benzoate were shown to have a very low cytotoxic activity. Taken together, our results suggest that IMU induces programed cell death in human fibroblasts by a canonical intrinsic pathway via mitochondrial perturbation and subsequent release of proapoptotic factors.
Keywords Preservatives
Pharmaceutical/chemistry
Fibroblasts/cytotoxicity
Cell death/drag effects
Cosmetics/additives
xmlui.dri2xhtml.METS-1.0.item-coverage Sao Paulo
Language English
Sponsor Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
CAPES
Date 2018
Published in Brazilian Journal Of Pharmaceutical Sciences. Sao Paulo, v. 54, n. 1, p. -, 2018.
ISSN 1984-8250 (Sherpa/Romeo, impact factor)
Publisher Univ Sao Paulo, Conjunto Quimicas
Extent -
Origin http://dx.doi.org/10.1590/s2175-97902018000100031
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000432451200001
SciELO ID S1984-82502018000100603 (statistics in SciELO)
URI https://repositorio.unifesp.br/handle/11600/54312

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