Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal

Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal

Author de Macedo, Erenildo Ferreira Autor UNIFESP Google Scholar
Ducatti Formaggio, Daniela Maria Autor UNIFESP Google Scholar
Santos, Nivia Salles Autor UNIFESP Google Scholar
Tada, Dayane Batista Autor UNIFESP Google Scholar
Abstract Although several researchers had reported on methodologies for surface plasmon resonance (SPR) signal amplification based on the use of nanoparticles (NPs), the majority addressed the sandwich technique and low protein concentration. In this work, a different approach for SPR signal enhancement based on the use of gold NPs was evaluated. The method was used in the detection of two lectins, peanut agglutinin (PNA) and concanavalin A (ConA). Gold NPs were functionalized with antibodies anti-PNA and anti-ConA, and these NPs were used as protein scavengers in a solution. After being incubated with solutions of PNA or ConA, the gold NPs coupled with the collected lectins were injected on the sensor containing the immobilized antibodies. The signal amplification provided by this method was compared to the signal amplification provided by the direct coupling of PNA and ConA to gold NPs. Furthermore, both methods, direct coupling and gold NPs as protein scavengers, were compared to the direct detection of PNA and ConA in solution. Compared to the analysis of free protein, the direct coupling of PNA and ConA to gold NPs resulted in a signal amplification of 10-40-fold and a 13-fold decrease of the limit of detection (LOD), whereas the use of gold NPs as protein scavengers resulted in an SPR signal 40-50-times higher and an LOD 64-times lower.
Keywords surface plasmon resonance
gold nanoparticles
signal amplification
lectins
concanavalin A
peanut agglutinin
xmlui.dri2xhtml.METS-1.0.item-coverage Basel
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/23895-8, 2017/01697-6]
Grant number FAPESP [2011/23895-8, 2017/01697-6]
Date 2017
Published in Sensors. Basel, v. 17, n. 12, p. -, 2017.
ISSN 1424-8220 (Sherpa/Romeo, impact factor)
Publisher Mdpi Ag
Extent -
Origin http://dx.doi.org/10.3390/s17122765
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000423285800065
URI https://repositorio.unifesp.br/handle/11600/54023

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