A reversible thermoresponsive sealant for temporary closure of ocular trauma

A reversible thermoresponsive sealant for temporary closure of ocular trauma

Author Bayat, Niki Google Scholar
Zhang, Yi Google Scholar
Falabella, Paulo Autor UNIFESP Google Scholar
Menefee, Roby Google Scholar
Whalen, John J., III Google Scholar
Humayun, Mark S. Google Scholar
Thompson, Mark E. Google Scholar
Abstract Open globe injuries are full-thickness injuries sustained to the eye wall (cornea or sclera), which cause immediate drops in intraocular pressure that may lead to retinal detachment and permanent vision loss if not treated rapidly after injury. The current standard of care for open globe injuries consists of suturing the margins closed, but the technique can be time-consuming, requires specialized training and equipment, and can lead to patient discomfort, abrasion, and infection from eye rubbing. We engineered an injectable, thermoresponsive sealant (TRS) and a custom tool to occlude open globe injuries. The smart hydrogel sealant consists of physically cross-linked N-iso-propylacrylamide copolymerized with butylacrylate. At low temperatures, it can be injected as a liquid, and when raised to body temperature, a heat-induced gelation converts the hydrogel into a solidified occlusion. The sealant can be repositioned or removed without causing additional trauma via exposure to cold water. In vitro and ex vivo assessments of mechanical adhesion to eye tissue revealed maintenance of intraocular pressure that is five times greater than the physiological range with reversible seal strength comparable to cyanoacrylate (super glue). In vivo assessment in a rabbit model of ocular trauma demonstrated ease of use for TRS deployment, statistically significant improvement in wound sealing, and no evidence of neurotoxicity, retinal tissue degradation, or significant chronic inflammatory response after 30 days of exposure. Given the advantages of body heat-induced gelation, rapid reversible occlusion, and in vivo safety and efficacy, shape-adaptable TRSs have translational potential as smart wound sealants for temporary occlusion of surgical incisions or traumatic injuries.
xmlui.dri2xhtml.METS-1.0.item-coverage Washington
Language English
Sponsor Office of the Assistant Secretary of Defense for Health Affairs through FY12 Vision Research Program [W81XWH12-1-0314]
NIH through SC-CTSI program [NIH/National Center for Research Resources (NCRR)/National Center for Advancing Translational Science (NCATS)] from NCATS [UL1TR000130, UL1TR001855]
NSF [EEC-0310723]
Research to Prevent Blindness
Date 2017
Published in Science Translational Medicine. Washington, v. 9, n. 419, p. -, 2017.
ISSN 1946-6234 (Sherpa/Romeo, impact factor)
Publisher Amer Assoc Advancement Science
Extent -
Origin http://dx.doi.org/10.1126/scitranslmed.aan3879
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000417196900003
URI https://repositorio.unifesp.br/handle/11600/54007

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