CD4(+) T helper cells and regulatory T cells in active lupus nephritis: an imbalance towards a predominant Th1 response?

CD4(+) T helper cells and regulatory T cells in active lupus nephritis: an imbalance towards a predominant Th1 response?

Author Mesquita, D., Jr. Google Scholar
Mastroianni Kirsztajn, G. Google Scholar
Franco, M. F. Autor UNIFESP Google Scholar
Reis, L. A. Google Scholar
Perazzio, S. F. Google Scholar
Mesquita, F. V. Google Scholar
da Silva Ferreira, V. Google Scholar
Coelho Andrade, L. E. Google Scholar
Silva de Souza, A. W. Google Scholar
Abstract The objective of this study was to evaluate the frequency of CD4(+) T cell subsets in peripheral blood mononuclear cells (PBMC), urine and renal tissue from patients with lupus nephritis (LN). PBMC and urinary cells were collected from 17 patients with active LN, 20 disease controls (DC) with primary glomerulonephritis and 10 healthy controls (HC) and were analysed by flow cytometry with markers for T helper type 1 (Th1), Th2, Th17 and regulatory T cells (T-reg) cells. T cell subsets were assessed by immunohistochemistry from LN biopsy specimens from 12 LN patients. T cell subtypes in PBMC were re-evaluated at 6 months of therapy. CD4(+) T cells were decreased in PBMC in LN compared with DC and HC (P=0<bold></bold>0001). No differences were observed in urinary CD4(+) T cell subsets between LN and DC. The frequency of urinary Th17 cells was higher in patients with non-proliferative than in proliferative LN (P=0<bold></bold>041). CD3(+) and T-box 21 ( <mml:msubsup>Tbet+</mml:msubsup>) cells were found in glomeruli and interstitium of LN patients, while forkhead box protein 3 (FoxP3), retinoid-related orphan receptor gamma (ROR-) and GATA binding protein 3 (GATA-3) were present only in glomeruli. Th1 cells in PBMC were correlated negatively with urinary Th1 cells (Rho=-0<bold></bold>531

P=0<bold></bold>028) and with T-bet in renal interstitium (Rho=-0<bold></bold>782

P=0<bold></bold>004). At 6 months, LN patients showed an increase in Th17 cells in PBMC. In conclusion, the inverse association between Th1 cells from PBMC and urinary/renal tissue indicate a role for Th1 in LN pathophysiology. Urinary Th17 cells were associated with less severe LN, and Th17 increased in PBMC during therapy. Urinary CD4(+) T cells were not different between LN and DC.
Keywords glomerulonephritis
helper T cells
lupus nephritis
regulatory T cells
systemic lupus erythematosus
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [10/51088-7]
Grant number FAPESP [10/51088-7]
Date 2018
Published in Clinical And Experimental Immunology. Hoboken, v. 191, n. 1, p. 50-59, 2018.
ISSN 0009-9104 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent 50-59
Origin http://dx.doi.org/10.1111/cei.13050
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000417564300006
URI https://repositorio.unifesp.br/handle/11600/53971

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