Collagen peptides modulate the metabolism of extracellular matrix by human dermal fibroblasts derived from sun-protected and sun-exposed body sites

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dc.contributor.author Zague, Vivian
dc.contributor.author do Amaral, Jonatas Bussador [UNIFESP]
dc.contributor.author Rezende-Teixeira, Paula
dc.contributor.author de Oliveira Niero, Evandro Luis
dc.contributor.author Lauand, Camila
dc.contributor.author Machado-Santelli, Glaucia Maria
dc.date.accessioned 2020-07-02T18:52:10Z
dc.date.available 2020-07-02T18:52:10Z
dc.date.issued 2018
dc.identifier http://dx.doi.org/10.1002/cbin.10872
dc.identifier.citation Cell Biology International. Hoboken, v. 42, n. 1, p. 95-104, 2018.
dc.identifier.issn 1065-6995
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/53915
dc.description.abstract Clinical data published in recent years have demonstrated positive effects of collagen hydrolysate (CH) on skin aging clinical signs. CH use as food supplement has a long history en
dc.description.abstract however, few studies have addressed the underlying purpose of CH on the cellular and molecular biology of skin cells that could elucidate clinical improvement findings. Wide diversity of characteristics has been reported for dermal fibroblasts derived from different body sites and it is unknown whether collagen peptides could modulate differently cells from chronological aged and photoaged skin areas. This study investigated the influence of CH on the extracellular matrix metabolism and proliferation of human dermal fibroblasts (HDFs) derived from chronological aged (sun-protected) and photoaged (sun-exposed) body sites. CH treatment did not affect cellular proliferation of either cell cultures, but notably modulated cell metabolism in monolayer model, increasing the content of dermal matrix precursor and main protein, procollagen I and collagen I, respectively. These effects were confirmed in the human dermal equivalent model. The increase in collagen content in the cultures was attributed to stimulation of biosynthesis and decreased collagen I metabolism through inhibition of metalloproteinase activity (MMP) 1 and 2. Modulation of CH in dermal metabolism did not differ between cells derived from sun-protected and sun-exposed areas, although lower concentrations of CH seemed to be enough to stimulate sun-exposed-derived HDFs, suggesting more pronounced effect in these cells. This study contributes to understanding the biological effects of CH on skin cells and viability of its use as a functional ingredient in food supplements. en
dc.description.sponsorship GELITA do Brasil Ltda
dc.description.sponsorship FAPESP
dc.description.sponsorship CAPES
dc.description.sponsorship CNPq
dc.format.extent 95-104
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartof Cell Biology International
dc.rights Acesso aberto
dc.subject collagen hydrolysate en
dc.subject dermal equivalent en
dc.subject ECM synthesis en
dc.subject MMP-1 activity en
dc.subject photoaged en
dc.subject skin aging en
dc.title Collagen peptides modulate the metabolism of extracellular matrix by human dermal fibroblasts derived from sun-protected and sun-exposed body sites en
dc.type Artigo
dc.description.affiliation Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Av Prof Lineu Prestes 1524, BR-05508000 Sao Paulo, SP, Brazil
dc.description.affiliation Univ Fed Sao Paulo, ENT Res Lab, Dept Otorhinolaryngol Head & Neck Surg, Rua Coronel Lisboa 958, BR-04023900 Sao Paulo, SP, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, ENT Res Lab, Dept Otorhinolaryngol Head & Neck Surg, Rua Coronel Lisboa 958, BR-04023900 Sao Paulo, SP, Brazil
dc.identifier.file WOS000422840600012.pdf
dc.identifier.doi 10.1002/cbin.10872
dc.description.source Web of Science
dc.identifier.wos WOS:000422840600012
dc.coverage Hoboken
dc.citation.volume 42
dc.citation.issue 1



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