Dual p16 and Ki-67 Expression in Liquid-Based Cervical Cytological Samples Compared to Pap Cytology Findings, Biopsies, and HPV Testing in Cervical Cancer Screening: A Diagnostic Accuracy Study

Dual p16 and Ki-67 Expression in Liquid-Based Cervical Cytological Samples Compared to Pap Cytology Findings, Biopsies, and HPV Testing in Cervical Cancer Screening: A Diagnostic Accuracy Study

Author Kabbach Prigenzi, Karla Calaca Autor UNIFESP Google Scholar
Heinke, Thais Autor UNIFESP Google Scholar
Salim, Rafael Calil Autor UNIFESP Google Scholar
de Azevedo Focchi, Gustavo Rubino Autor UNIFESP Google Scholar
Abstract Objective: Our objective was to verify the sensitivity and specificity of dual immunocytochemistry staining for p16 and Ki-67 in liquid-based samples (the "dual" assay) for cervical lesion screening, compared to biopsy findings and human papillomavirus (HPV) DNA molecular detection. Study Design: Sensitivity, specificity, and positive (PPV) and negative (NPV) predictive values for the "dual immunocytochemistry assay" were calculated and compared to histopathological results and to high-risk HPV DNA detection in adult women or teenagers submitted to cervical cancer screening. Results: A total of 151 women were included. The majority (96.2%) of those with negative dual assay results had lower biopsy grades (p < 0.001). Women with cytology results suggestive of cervical cancer had positive dual immunocytochemistry assay results more frequently (p < 0.001), and these positive results were also significantly associated with biopsy findings (p < 0.001) and with high-risk genotype HPV infection (p = 0.007). Specificity and PPV for the dual assay were 0.972 (0.855-0.999) and 0.800 (0.284-0.995), respectively, and 1.000 (0.590-1.000) and 1.000 (0.631-1.000) for HPV detection. Conclusions: The dual immunocytochemistry assay had high specificity and PPV. It reveals a persistent HPV infection, avoiding the need for new tissue collections for biopsies or hybrid capture. (C) 2018 S. Karger AG, Basel
Keywords Cervical intraepithelial neoplasia
Uterine cervical neoplasms
Uterine cervical dysplasia
Immunocytochemistry
Papanicolaou test
Cytological techniques
Cyclin-dependent kinase inhibitor p16
Genes
Ki-67 antigen
Diagnostic techniques
xmlui.dri2xhtml.METS-1.0.item-coverage Basel
Language English
Sponsor CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
Date 2018
Published in Acta Cytologica. Basel, v. 62, n. 2, p. 104-114, 2018.
ISSN 0001-5547 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 104-114
Origin http://dx.doi.org/10.1159/000487504
Access rights Closed access
Type Article
Web of Science ID WOS:000431129100005
URI https://repositorio.unifesp.br/handle/11600/53742

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