Sleep restriction in Wistar rats impairs epididymal postnatal development and sperm motility in association with oxidative stress

Sleep restriction in Wistar rats impairs epididymal postnatal development and sperm motility in association with oxidative stress

Author Siervo, Glaucia E. M. L. Google Scholar
Ogo, Fernanda M. Google Scholar
Valerio, Aline D. Google Scholar
Silva, Thamara N. X. Google Scholar
Staurengo-Ferrari, Larissa Google Scholar
Alvarenga, Tathiana A. Autor UNIFESP Google Scholar
Cecchini, Rubens Google Scholar
Verri, Waldiceu A., Jr. Google Scholar
Guarnier, Flavia A. Google Scholar
Andersen, Monica L. Autor UNIFESP Google Scholar
Fernandes, Glaura S. A. Google Scholar
Abstract Good sleep quality has a direct effect on the activity of the neuroendocrine-reproductive control axis and oxidative stress. Thus, the aim of the present study was to evaluate whether sleep restriction (SR) during the peripubertal period impaired the postnatal development of the epididymis in Wistar rats. After 21 days SR (18h per day), epididymides were collected on Postnatal Day (PND) 62 for evaluation of oxidative stress markers, inflammatory profile, sperm count and histopathological and stereological analyses

in addition, the motility of spermatozoa from the vas deferens was examined. SR significantly increased lipid peroxidation and glutathione levels in the caput and cauda epididymidis, and increased levels of total radical-trapping antioxidant potential in the caput epididymidis only. Neutrophil migration to the caput or corpus epididymidis was decreased by SR, and the size of the luminal compartment in the 2A region and the epithelial compartment in the 5A/B region was also decreased. In these regions, there was an increase in the size of the interstitial compartment. The percentage of immotile spermatozoa was higher in the SR group. In conclusion, SR affects epididymal postnatal development, as well as sperm motility, in association with increased oxidative stress and a decrease in the size of the epithelial compartment in the cauda epididymidis.
Language English
Sponsor CAPES - PROEX (Coordinating Body for the Improvement of Postgraduate Studies in Higher Education, Brazil)
FAPESP (State of Sao Paulo Research Foundation, Brazil)
CNPq (National Council for Scientific and Technological Development, Brazil)
Grant number FAPESP: 11/12325-6
Date 2017
Published in Reproduction Fertility And Development. Clayton, v. 29, n. 9, p. 1813-1820, 2017.
ISSN 1031-3613 (Sherpa/Romeo, impact factor)
Publisher Csiro Publishing
Extent 1813-1820
Access rights Closed access
Type Article
Web of Science ID WOS:000407701500015

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