Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum

Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum

Author de Almeida, Joao N., Jr. Google Scholar
Favero Gimenes, Viviane M. Google Scholar
Francisco, Elaine C. Autor UNIFESP Google Scholar
Machado Siqueira, Lumena P. Google Scholar
Goncalves de Almeida, Renato K. Google Scholar
Guitard, Juliette Google Scholar
Hennequin, Christophe Google Scholar
Colombo, Arnaldo L. Autor UNIFESP Google Scholar
Benard, Gil Google Scholar
Rossi, Flavia Google Scholar
Abstract Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immuno-compromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trichosporon taxonomy has been reassessed, and three genera have been proposed for the pathogenic species: Trichosporon, Cutaneotrichosporon, and Apiotrichum. Matrix-assisted laser desorption ionization- time of flight mass spectrometry (MALDI-TOF MS) has replaced old phenotypic methods for microorganism identification in clinical laboratories, but spectral profile databases have to be evaluated and improved for optimal species identification performance. Vitek MS (bioMerieux) is one of the commercially available MALDI-TOF MS platforms for pathogen identification, and its spectral profile databases remain poorly evaluated for Trichosporon, Cutaneotrichosporon, and Apiotrichum species identification. We herein evaluated and improved Vitek MS for the identification of the main clinical relevant species of Trichosporon, Cutaneotrichosporon, and Apiotrichum using a large set of strains and isolates belonging to different yeast collections in Brazil and France.
Keywords Trichosporon
Cutaneotrichosporon
Apiotrichum
Vitek M
MALDI-TOF mass spectrometry
Language English
Sponsor National Council of Technological and Scientific Development (CNPq)
Grant number CNPq: 307510/2015-8
Date 2017
Published in Journal Of Clinical Microbiology. Washington, v. 55, n. 8, p. 2439-2444, 2017.
ISSN 0095-1137 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 2439-2444
Origin http://dx.doi.org/10.1128/JCM.00461-17
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000408109800019
URI http://repositorio.unifesp.br/handle/11600/51441

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