GTSP1 expression in non-smoker and nondrinker patients with squamous cell carcinoma of the head and neck

GTSP1 expression in non-smoker and nondrinker patients with squamous cell carcinoma of the head and neck

Author Soares, Pamela de Oliveira Google Scholar
Cury, Patricia Maluf Google Scholar
Mendoza Lopez, Rossana Veronica Google Scholar
Cernea, Claudio Roberto Google Scholar
Fukuyama, Erika Erina Google Scholar
Alves Figueiredo, David Livingstone Google Scholar
da Nobrega, Francisco Gorgonio Autor UNIFESP Google Scholar
Curioni, Otavio Alberto Google Scholar
Nunes, Fabio Daumas Google Scholar
Moyses, Raquel Ajub Google Scholar
Bueno Garcia, Maria Lucia Google Scholar
Abstract Introduction The main risk factors for head and neck squamous cell carcinoma (HNSCC) are tobacco and alcohol consumption and human papillomavirus (HPV) infection. However, in a subset of patients, no risk factors can be identified. Glutathione S-transferase p (GTSP1) is a carcinogen-detoxifying enzyme that is activated by exposure to carcinogens, and it is associated with a reduction in response to toxic therapies. We studied the expression of GTSP1 in tumor and non-tumor tissue samples from patients with and without these risks to identify whether GTSP1 expression differs according to exposure to carcinogens. Materials and methods Non-smoker/non-drinker (NSND) and smoker/drinker (SD) patients were matched according to age, gender, tumor site, TNM stage, grade and histological variants to establish 47 pairs of patients who have been previously tested for HPV. GTSP1 immunostaining was analyzed using a semi-quantitative method with scores ranging from 0 to 3 according to the area of immunostaining. Results GTSP1 expression was detected in the tumors of both groups. GTSP1 expression was higher in the non-tumor margins of SD patients (p = 0.004). There was no association between GTSP1 expression and positivity for HPV. No differences in survival were observed according to GTSP1 staining in tumors and non-tumor margins. Conclusion This study showed that GTSP1 was expressed in tumors of HNSCC patients regardless of smoking, drinking or HPV infection status. The difference in GTSP1 expression in non-tumor margins between the two groups may have been due to two possible reasons. First, elevated GTSP1 expression in SD patients might be the result of activation of GTSP1 in response to exposure to carcinogens. Second, alternatively, impairment in the detoxifying system of GTSP1, as observed by the reduced expression of GTSP1, might make patients susceptible to carcinogens other than tobacco and alcohol, which may be the underlying mechanism of carcinogenesis in the absence of risk factors.
Language English
Sponsor Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Research scholarship to Pamela de Oliveira Soares
Grant number FAPESP: 04/12054-9
Date 2017
Published in Plos One. San Francisco, v. 12, n. 8, p. -, 2017.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent -
Origin http://dx.doi.org/10.1371/journal.pone.0182600
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000407856600033
URI http://repositorio.unifesp.br/handle/11600/51384

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