First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (acai) in cultured human cells

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dc.contributor.author Marques, E. S.
dc.contributor.author Tsuboy, M. S. F.
dc.contributor.author Carvalho, J. C. T.
dc.contributor.author Rosa, P. C. P.
dc.contributor.author Perazzo, F. F. [UNIFESP]
dc.contributor.author Gaivao, I. O. M.
dc.contributor.author Maistro, E. L.
dc.date.accessioned 2019-08-19T11:49:42Z
dc.date.available 2019-08-19T11:49:42Z
dc.date.issued 2017
dc.identifier http://dx.doi.org/10.4238/gmr16039700
dc.identifier.citation Genetics And Molecular Research. Ribeirao Preto, v. 16, n. 3, p. -, 2017.
dc.identifier.issn 1676-5680
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/51382
dc.description.abstract Euterpe oleracea Mart., popularly known as "acai", is a tropical fruit from the Amazon region where it has considerable economic importance. Acai has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and antigenotoxic effects of E. oleracea fruit oil (EOO) in cultured human lymphocytes (non-metabolizing cells) and HepG2 cell line (human hepatoma) (metabolizing cells) by using MTT, comet, and micronucleus assays. A wide range of EOO concentrations was tested with a preliminary MTT assay, which allowed selecting five concentrations for comet and micronucleus assays: 2.5, 10, 100, 500, and 1000 mu g/mL. The results showed that none of the EOO tested concentrations presented cytotoxic effects. The genotoxic assessment revealed an absence of significant DNA and chromosome damage in human lymphocytes and HepG2 cells but did not show chemoprotection against the DNA damage induced by methyl methanesulfonate and benzo[a] pyrene, used as DNA-damaging agents. en
dc.description.sponsorship FAPESP - Fundação de Amparo a Pesquisa do Estado de São Paulo
dc.description.sponsorship CAPES
dc.format.extent -
dc.language.iso eng
dc.publisher Funpec-Editora
dc.rights Acesso restrito
dc.subject Acai oil en
dc.subject Arecaceae en
dc.subject Comet assay en
dc.subject HepG2 cells en
dc.subject Micronucleus test en
dc.title First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (acai) in cultured human cells en
dc.type Artigo
dc.description.affiliation Univ Estadual Paulista, Inst Biociencias, Programa Posgrad Biol Geral & Aplicada, Botucatu, SP, Brazil
dc.description.affiliation Univ Estadual Paulista, Fac Ciencias & Letras, Dept Biotecnol, Assis, SP, Brazil
dc.description.affiliation Univ Fed Amapa, Colegiado Farm, Ctr Ciencias Biol & Saude, Lab Pesquisa Farmacos, Macapa, AP, Brazil
dc.description.affiliation Univ Estadual Campinas, Fac Ciencias Med, Campinas, SP, Brazil
dc.description.affiliation Univ Fed São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, Diadema, SP, Brazil
dc.description.affiliation Tras Os Montes & Alto Douro Univ, CECAV, Vila Real, Portugal
dc.description.affiliation Tras Os Montes & Alto Douro Univ, Dept Genet & Biotechnol, Vila Real, Portugal
dc.description.affiliation Univ Estadual Paulista, Fac Filosofia & Ciencia, Dept Fonoaudiol, Marilia, SP, Brazil
dc.description.affiliationUnifesp Univ Fed São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, Diadema, SP, Brazil
dc.description.sponsorshipID FAPESP: 2012/17241-8
dc.identifier.doi 10.4238/gmr16039700
dc.description.source Web of Science
dc.identifier.wos WOS:000417364500008



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