First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (acai) in cultured human cells

First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (acai) in cultured human cells

Author Marques, E. S. Google Scholar
Tsuboy, M. S. F. Google Scholar
Carvalho, J. C. T. Google Scholar
Rosa, P. C. P. Google Scholar
Perazzo, F. F. Autor UNIFESP Google Scholar
Gaivao, I. O. M. Google Scholar
Maistro, E. L. Google Scholar
Abstract Euterpe oleracea Mart., popularly known as "acai", is a tropical fruit from the Amazon region where it has considerable economic importance. Acai has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and antigenotoxic effects of E. oleracea fruit oil (EOO) in cultured human lymphocytes (non-metabolizing cells) and HepG2 cell line (human hepatoma) (metabolizing cells) by using MTT, comet, and micronucleus assays. A wide range of EOO concentrations was tested with a preliminary MTT assay, which allowed selecting five concentrations for comet and micronucleus assays: 2.5, 10, 100, 500, and 1000 mu g/mL. The results showed that none of the EOO tested concentrations presented cytotoxic effects. The genotoxic assessment revealed an absence of significant DNA and chromosome damage in human lymphocytes and HepG2 cells but did not show chemoprotection against the DNA damage induced by methyl methanesulfonate and benzo[a] pyrene, used as DNA-damaging agents.
Keywords Acai oil
Arecaceae
Comet assay
HepG2 cells
Micronucleus test
Language English
Sponsor FAPESP - Fundação de Amparo a Pesquisa do Estado de São Paulo
CAPES
Grant number FAPESP: 2012/17241-8
Date 2017
Published in Genetics And Molecular Research. Ribeirao Preto, v. 16, n. 3, p. -, 2017.
ISSN 1676-5680 (Sherpa/Romeo, impact factor)
Publisher Funpec-Editora
Extent -
Origin http://dx.doi.org/10.4238/gmr16039700
Access rights Closed access
Type Article
Web of Science ID WOS:000417364500008
URI http://repositorio.unifesp.br/handle/11600/51382

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