Hippocampal Proteome of Rats Subjected to the Li-Pilocarpine Epilepsy Model and the Effect of Carisbamate Treatment

Hippocampal Proteome of Rats Subjected to the Li-Pilocarpine Epilepsy Model and the Effect of Carisbamate Treatment

Author Marques-Carneiro, Jose Eduardo Autor UNIFESP Google Scholar
Persike, Daniele Suzete Autor UNIFESP Google Scholar
Litzahn, Julia Julie Autor UNIFESP Google Scholar
Cassel, Jean-Christophe Google Scholar
Nehlig, Astrid Google Scholar
da Silva Fernandes, Maria Jose Autor UNIFESP Google Scholar
Abstract In adult rats, the administration of lithium-pilocarpine (LiPilo) reproduces most clinical and neuropathological features of human temporal lobe epilepsy (TLE). Carisbamate (CRS) possesses the property of modifying epileptogenesis in this model. Indeed, about 50% of rats subjected to LiPilo status epilepticus (SE) develop non-convulsive seizures (NCS) instead of motor seizures when treated with CRS. However, the mechanisms underlying these effects remain unknown. The aim of this study was to perform a proteomic analysis in the hippocampus of rats receiving LiPilo and developing motor seizures or NCS following CRS treatment. Fifteen adult male Sprague-Dawley rats were used. SE was induced by LiPilo injection. CRS treatment was initiated at 1 h and 9 h after SE onset and maintained for 7 days, twice daily. Four groups were studied after video-EEG control of the occurrence of motor seizures: a control group receiving saline (CT n = 3) and three groups that underwent SE: rats treated with diazepam (DZP n = 4), rats treated with CRS displaying NCS (CRS-NCS n = 4) or motor seizures (CRS-TLE n = 4). Proteomic analysis was conducted by 2D-SDS-PAGE. Twenty-four proteins were found altered. In the CRS-NCS group, proteins related to glycolysis and ATP synthesis were down-regulated while proteins associated with pyruvate catabolism were up-regulated. Moreover, among the other proteins differentially expressed, we found proteins related to inflammatory processes, protein folding, tissue regeneration, response to oxidative stress, gene expression, biogenesis of synaptic vesicles, signal transduction, axonal transport, microtubule formation, cell survival, and neuronal plasticity. Our results suggest a global reduction of glycolysis and cellular energy production that might affect brain excitability. In addition, CRS seems to modulate proteins related to many other pathways that could significantly participate in the epileptogenesis-modifying effect observed.
Keywords carisbamate
temporal-lobe epilepsy
hippocampus
proteomics
brain activity
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo a Pesquisa no Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Apoio a Unifesp (FAP-UNIFESP)
Date 2017
Published in Pharmaceuticals. Basel, v. 10, n. 3, p. -, 2017.
ISSN 1424-8247 (Sherpa/Romeo, impact factor)
Publisher Mdpi Ag
Extent -
Origin http://dx.doi.org/10.3390/ph10030067
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000411351400010
URI http://repositorio.unifesp.br/handle/11600/51293

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