Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection

Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection

Alternative title Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection
Author Paulini, Inarei Autor UNIFESP Google Scholar
Siqueira-Silva, Joselma Google Scholar
Thomaz, Luciana Google Scholar
Rocha, Leticia Google Scholar
Harsi, Charlotte Google Scholar
Bellei, Nancy Autor UNIFESP Google Scholar
Granato, Celso Autor UNIFESP Google Scholar
Abstract Human adenoviruses comprise an important group of etiologie agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.& para;& para;The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.& para;& para;Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods. Antibodies were specific for adenoviruses 2,3,5, and 41. The proposed immunochromatographic test was standardized using colloidal gold.& para;& para;The standardization of the rapid test was sufficient to detect adenovirus antigens (in nasopharyngeal lavage samples) with sensitivity of 100% and specificity of 85% when compared to direct immunofluorescence.& para;& para;The immunochromatographic assay prototype was sufficiently sensitive to detect B (3), C (2 and 5), and F (41) adenovirus samples. Although based on preliminary data, the test demonstrated the same performance as direct immunofluorescence, but with the advantage of being a point-of-care test. Further studies are still needed to confirm its effectiveness in clinical practice. (C) 2017 Sociedade Brasueira de Infectologia. Published by Elsevier Editera Ltda.
Keywords Adenovirus
Monoclonal antibodies
Immunochromatographic test
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2011/50100-6
Date 2017
Published in Brazilian Journal Of Infectious Diseases. Rio De Janeiro, v. 21, n. 5, p. 500-506, 2017.
ISSN 1413-8670 (Sherpa/Romeo, impact factor)
Publisher Elsevier Brazil
Extent 500-506
Origin http://dx.doi.org/10.1016/j.bjid.2017.03.023
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000414276300002
SciELO ID S1413-86702017000500500 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/51258

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