Novel immunoassay for TSH measurement in rats

Novel immunoassay for TSH measurement in rats

Author Alves, Thalita G. Autor UNIFESP Google Scholar
Melo, Maria Clara de C. Autor UNIFESP Google Scholar
Kasamatsu, Teresa S. Autor UNIFESP Google Scholar
Oliveira, Kelen C. Autor UNIFESP Google Scholar
de Souza, Janaina Sena Autor UNIFESP Google Scholar
da Conceicao, Rodrigo Rodrigues Autor UNIFESP Google Scholar
Giannocco, Gisele Autor UNIFESP Google Scholar
Dias-da-Silva, Magnus R. Autor UNIFESP Google Scholar
Chiamolera, Maria Izabel Autor UNIFESP Google Scholar
Vieira, Jose Gilberto Autor UNIFESP Google Scholar
Abstract Measuring thyroid hormones is an important aspect for the study of metabolism and for monitoring diseases in both human and animal models. The traditional method for hormone measurement in rats is the radioimmunoassay (RIA). However, the RIA is associated with some practical disadvantages, including the use of radioactive material, the need for specialized equipment and expert staff, the short shelf-life of kits according to the half-life of the radioisotope and high costs. The objective of this study was to develop a new cost-effective method for measuring TSH levels in rats that avoids the use of radioactive material. We developed an in-house competitive immunoassay using a reference standard, polyclonal antibody produced in rabbits and biotinylated antigen. This method was tested in 64 Wistar rats that were divided into a control group (n = 41) and a group with hypothyroidism (n = 23). Our assay demonstrated an analytical sensitivity of 0.24 ng/mL (n = 12) and an intra-assay coefficient of variation (CV) of 8.9% for sera with TSH levels of 1.5 ng/mL and 13.2% for sera with TSH levels of 17.5 ng/mL (n = 14). The inter-assay CV was 13.5% for sera with TSH levels of 1.4 ng/mL and 14.5% for TSH levels of 18.2 ng/mL (n = 5). The analysis of mean TSH levels in control rats (5.06 +/- 0.5701) and hypothyroid rats (51.09 +/- 5.136) revealed a statistically significant difference (p < 0.001) between the groups. This method showed good sensitivity, can be automated and is low-cost compared with RIA. Our method offers a viable alternative for TSH measurement in rats.
Keywords Immunoassay
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2013/26851-7
Date 2017
Published in Archives Of Endocrinology Metabolism. Rio De Janeiro, Rj, v. 61, n. 5, p. 460-463, 2017.
ISSN 2359-3997 (Sherpa/Romeo, impact factor)
Publisher Sbem-Soc Brasil Endocrinologia & Metabologia
Extent 460-463
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000416002900009
SciELO ID S2359-39972017000500460 (statistics in SciELO)

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