AC-1001 H3 CDR peptide induces apoptosis and signs of autophagy in vitro and exhibits antimetastatic activity in a syngeneic melanoma model

AC-1001 H3 CDR peptide induces apoptosis and signs of autophagy in vitro and exhibits antimetastatic activity in a syngeneic melanoma model

Author Rabaca, Aline N. Autor UNIFESP Google Scholar
Arruda, Denise C. Autor UNIFESP Google Scholar
Figueiredo, Carlos R. Autor UNIFESP Google Scholar
Massaoka, Mariana H. Autor UNIFESP Google Scholar
Farias, Camyla F. Autor UNIFESP Google Scholar
Tada, Dayane B. Autor UNIFESP Google Scholar
Maia, Vera C. Google Scholar
Silva Junior, Pedro I. Google Scholar
Girola, Natalia Autor UNIFESP Google Scholar
Real, Fernando Autor UNIFESP Google Scholar
Mortara, Renato A. Autor UNIFESP Google Scholar
Polonelli, Luciano Google Scholar
Travassos, Luiz R. Autor UNIFESP Google Scholar
Abstract Antibody-derived peptides modulate functions of the immune system and are a source of anti-infective and antitumor substances. Recent studies have shown that they comprise amino acid sequences of immunoglobulin complementarity-determining regions, but also fragments of constant regions. V-H CDR3 of murine mAb AC-1001 displays antimetastatic activities using B16F10-Nex2 murine melanoma cells in a syngeneic model. The peptide was cytotoxic in vitro in murine and human melanoma cells inducing reactive oxygen species (ROS) and apoptosis by the intrinsic pathway. Signs of autophagy were also suggested by the increased expression of LC3/LC3II and Beclin 1 and by ultrastructural evidence. AC-1001 H3 bound to both G- and F-actin and inhibited tumor cell migration. These results are important evidence of the antitumor activity of Ig CDR-derived peptides.
Keywords apoptosis
autophagy
immunoglobulin CDR
melanoma
peptides
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2010/16447-6, 51423-0]
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Grant number FAPESP:2010/16447-6
51423-0
Date 2016
Published in Febs Open Bio. Hoboken, v. 6, n. 9, p. 885-901, 2016.
ISSN 2211-5463 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent 885-901
Origin http://dx.doi.org/10.1002/2211-5463.12080
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000383618300002
URI http://repositorio.unifesp.br/handle/11600/51077

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