Analysis of Intraocular Lens Biofilms and Fluids After Long-Term Uncomplicated Cataract Surgery

Analysis of Intraocular Lens Biofilms and Fluids After Long-Term Uncomplicated Cataract Surgery

Author Mazoteras, Paloma Google Scholar
Quiles, Milene Goncalves Autor UNIFESP Google Scholar
Martins Bispo, Paulo Jose Autor UNIFESP Google Scholar
Hofling-Lima, Ana Luisa Autor UNIFESP Google Scholar
Pignatari, Antonio C. Autor UNIFESP Google Scholar
Casaroli-Marano, Ricardo P. Google Scholar
Abstract PURPOSE: Postoperative endophthalmitis is a potentially sight-threatening complication of cataract surgery. However, the pathophysiological mechanisms are not completely understood. We sought to study and evaluate the intraocular environment (aqueous and vitreous humors), the capsular tissue, and the intraocular lens (IOL) surfaces of normal eyes after long-term uncomplicated cataract surgery. DESIGN: Experimental laboratory investigation. METHODS: We studied 69 eyes donated for transplantation that had previously undergone cataract surgery with posterior chamber IOL implantation and that had no recorded clinical history of postoperative inflammation. We assessed the intraocular environment (DNA traces and biofilm formation) by microbiological evaluation of intraocular fluids using conventional microbiology and molecular techniques, including assessment for the presence of microbes (biofilm formation) on the IOL surface by scanning electron microscopy and ultrastructural capsular remnants by transmission electron microscopy. RESULTS: Isolated or aggregated cocci were probable in 18.8% of IOL optic surfaces (n = 13) studied by scanning electron microscopy, suggesting the presence of bacterial biofilm. In 3 intraocular fluid samples for IOLs with biofilm, we identified 16S rDNA by polymerase chain reaction and sequencing. No microbial contamination was found in intraocular fluids by conventional microbiological methods. CONCLUSIONS: Our data suggest the possibility of bacterial biofilm formation on the optic surface of IOLs in normal eyes after long-term uncomplicated cataract surgery even in the absence of clinical or subclinical symptoms. (C) 2016 Elsevier Inc. All rights reserved.
Language English
Sponsor FONDOS DE INVESTIGACIONES SANITARIAS, Instituto de Salud Carlos III (Madrid, Spain)
Grant number ISCIII: PI09/0992
Date 2016
Published in American Journal Of Ophthalmology. New York, v. 169, p. 46-57, 2016.
ISSN 0002-9394 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Inc
Extent 46-57
Access rights Closed access
Type Article
Web of Science ID WOS:000383637300006

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