Immunohistochemical analysis of pdgfr-alpha, pdgfr-beta and c-abl in retinoblastoma: potential therapeutic targets

Immunohistochemical analysis of pdgfr-alpha, pdgfr-beta and c-abl in retinoblastoma: potential therapeutic targets

Author Sanft, Debra-Meghan Google Scholar
Worme, Mali Dawn Google Scholar
de Moura, Leticia Rielo Autor UNIFESP Google Scholar
Zoroquiain, Pablo Google Scholar
Fernandes, Bruno F. Google Scholar
Antecka, Emilia Google Scholar
Burnier, Miguel N., Jr. Google Scholar
Abstract Background: Our laboratory previously reported that imatinib mesylate (IM) has an inhibitory effect on two retinoblastoma (Rb) cell lines in vitro. Aims: The purpose of this project was to determine the immunoexpression of platelet-derived growth factor receptor (PDGFR)-alpha, PDGFR-beta and c-Abl in 61 human samples of Rb to determine if IM-sensitive receptors are present. Additionally, this paper seeks to establish a correlation between the expression of PDGFR, c-Abl and the histopathological prognosis. Methods: Sixty-one paraffin-embedded Rbs were collected from the Henry C. Witelson Ocular Pathology Registry. PDGFR-alpha, PDGFR-beta and c-Abl immunostaining was performed according to the protocol provided by Ventana Medical System Inc. Immunoreactivity was correlated with the presence or absence of invasion into the choroid and optic nerve. Results: Overall, c-Abl expression was identified in 50 out of 61 specimens (81.97%), PDGFR-alpha was identified in 20 out of 60 specimens (33.33%) and PDGFR-beta expression was identified in 57 out of 61 specimens (93.44%). Histopathological prognosis was not correlated with immunoreactivity except in the case of PDGFR-beta. Conclusions: Rb is a cancer that expresses PDGFR-alpha, PDGFR-beta and c-Abl, which are known targets of IM. These markers may be responsible for the documented therapeutic effect of IM on Rb cell lines. (C) 2016 S. Karger AG, Basel
Keywords Retinoblastoma
Platelet-Derived Growth Factor Receptor
ImmunohistochemistryReceptor Tyrosine Kinases
Imatinib Mesylate
Expression
Proliferation
Osteosarcoma
Tumors
Language English
Date 2016
Published in Ophthalmic Research. Basel, v. 55, n. 3, p. 159-162, 2016.
ISSN 0030-3747 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 159-162
Origin https://doi.org/10.1159/000442882
Access rights Closed access
Type Article
Web of Science ID WOS:000369673800007
URI http://repositorio.unifesp.br/handle/11600/49631

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