Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats

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dc.contributor.author Fiorindo Faria, Danilo Jose [UNIFESP]
dc.contributor.author Simoes, Manuel de Jesus [UNIFESP]
dc.contributor.author Teixeira, Luciana Cristina [UNIFESP]
dc.contributor.author Pinheiro Faria, Angela Tucci
dc.contributor.author Simoes Ulhoa Cintra, Alvaro Edmundo [UNIFESP]
dc.contributor.author Martins, Jose Luiz [UNIFESP]
dc.date.accessioned 2019-01-21T10:30:09Z
dc.date.available 2019-01-21T10:30:09Z
dc.date.issued 2016
dc.identifier http://dx.doi.org/10.1590/S0102-865020160010000004
dc.identifier.citation Acta Cirurgica Brasileira. Sao paulo, v. 31, n. 1, p. 22-27, 2016.
dc.identifier.issn 0102-8650
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/49619
dc.description.abstract PURPOSE: To determine the effect of a single dose of adriamycin (ADR) to induce anorectal malformations (ARMs) and determine the effect of folic acid (FA) in this model. METHODS: Ten female Wistar rats were divided randomly in two groups. Group A - ADR en
dc.description.abstract Group B - FA+ADR. Dams from group B received daily, since two weeks before the pregnancy to the end of pregnancy, FA (50mg/kg) by gavage. Dams from both groups received ADR (6mk/kg) by intraperitoneal injection on gestational day (GD) 8. Their fetuses were harvested by cesarean section on GD21 and were examined looking for ARMs. The thickness of anal stratified squamous epithelium (ASSE) and intestinal epithelium (IE) were analyzed. p <= 0.05*. RESULTS: 81 fetuses were harvested. The number of fetuses en
dc.description.abstract number of ARMs en
dc.description.abstract mean (Delta%) (+/- SD) were determined to be, respectively: ADR -41[29 en
dc.description.abstract 65%(+/- 37%)] versus FA+ADR - 40[04 en
dc.description.abstract 16%(+/- 36%)] (p=0.05). AMRs were significantly lower in FA+ADR group than in ADR group (p=0.05). The thickness (mu m) of ASSE (+/- SD) and IE (+/- SD) were measured, respectively: ADR -[25.98(+/- 0.74) and 19.48(+/- 1.68)] versus FA+ADR -[24.74(+/- 0.91) and 24.80(+/- 0.81)] (p<0.005). The thickness of IE was significantly enlarged when FA was given (p<0.005). CONCLUSIONS: Single dose of adriamycin on D8 was able to induce anorectal malformations. Folic acid reduces the number and enlarged the IE of ARMs ADR-induced. en
dc.format.extent 22-27
dc.language.iso eng
dc.publisher Acta cirurgica brasileira
dc.relation.ispartof Acta Cirurgica Brasileira
dc.rights Acesso aberto
dc.subject Anal Canal en
dc.subject Anus en
dc.subject Imperforate en
dc.subject Doxorubicin en
dc.subject Folic Acid en
dc.subject RatsNeural-Tube Defects en
dc.subject Embryonic-Development en
dc.subject Hindgut Development en
dc.subject Mouse Model en
dc.subject Supplementation en
dc.subject Notochord en
dc.subject Ethylenethiourea en
dc.subject Expression en
dc.subject Pregnancy en
dc.subject Anomalies en
dc.title Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats en
dc.type Artigo
dc.description.affiliation Postgraduate Program in Interdisciplinary Surgical Science, Universidade Federal de São Paulo (UNIFESP), Brazil
dc.description.affiliation Histology and Structural Biology Division, Department of Morphology, UNIFESP, Sao Paulo-SP, Brazil
dc.description.affiliation Universidade São Judas Tadeu, Sao Paulo-SP, Brazil
dc.description.affiliation Department of Pediatric Surgery, UNIFESP, Sao Paulo-SP, Brazil
dc.description.affiliationUnifesp Postgraduate Program in Interdisciplinary Surgical Science, Universidade Federal de São Paulo (UNIFESP), Brazil
dc.description.affiliationUnifesp Histology and Structural Biology Division, Department of Morphology, UNIFESP, Sao Paulo-SP, Brazil
dc.description.affiliationUnifesp Department of Pediatric Surgery, UNIFESP, Sao Paulo-SP, Brazil
dc.identifier.file S0102-86502016000100022.pdf
dc.identifier.scielo S0102-86502016000100022
dc.identifier.doi 10.1590/S0102-865020160010000004
dc.description.source Web of Science
dc.identifier.wos WOS:000369657900004



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