Analysis of polymorphisms in codons 11, 72 and 248 of tp53 in brazilian women with breast cancer

Analysis of polymorphisms in codons 11, 72 and 248 of tp53 in brazilian women with breast cancer

Author Almeida, B. C. Autor UNIFESP Google Scholar
Kleine, J. P. F. O. Autor UNIFESP Google Scholar
Camargo-Kosugi, C. M. Autor UNIFESP Google Scholar
Lisboa, M. R. Autor UNIFESP Google Scholar
Franca, C. N. Autor UNIFESP Google Scholar
Franca, J. P. Google Scholar
Silva, I. D. C. G. Autor UNIFESP Google Scholar
Abstract The association between TP53 gene polymorphisms and breast cancer (BC) in Brazilian women is a controversial topic. In this cross-sectional study, we evaluated the association between clinical pathological variables and three polymorphisms (TP53(star)11, TP53(star)72, and TP53(star)248) in BC patients and controls. Genomic DNA was extracted from the blood cells of 393 participants

the cancer-free control subjects were 26-72 years old (41 +/- 11.03) and the BC patients were 28-80 years old (51 +/- 10.70). We used standard polymerase chain reaction-restriction fragment length polymorphism and confirmed the results by genetic sequencing. In TP53(star)11, there was 100% homozygous Glu distribution in both groups. TP53(star)72 showed genotypic distribution: in the control group, there was 16.10% homozygous Pro, and 42.44% heterozygous and 41.46% homozygous Arg

in the BC group, there was 15.43% homozygous Pro, and 42.55% heterozygous and 42.02% homozygous Arg. The relative frequency of each allele was 0.37% for Pro and 0.63% for Arg in the control group, and 0.37% for Pro and 0.63% for Arg in the BC group. The nuclear grade (P = 0.0084) and adapted histological grade (P = 0.0265) were associated with TP53(star)72. The distribution of the codon 72 genotypes did not deviate from Hardy-Weinberg equilibrium in either group. In TP53(star)248, there was 100% homozygous Arg distribution in both groups. In codon 72, the Arg allele is the most prevalent in Brazilian women. TP53(star)72 may be associated with susceptibility to BC, although more studies are required to evaluate the profile of Brazilian women with BC.
Keywords Breast Cancer
Restriction Enzymes
P53 Protein
Brazilian WomenGene Polymorphisms
P53 Polymorphisms
Language English
Sponsor CNPq
Date 2016
Published in Genetics And Molecular Research. Ribeirao preto, v. 15, n. 1, p. UNSP gmr.15017055, 2016.
ISSN 1676-5680 (Sherpa/Romeo, impact factor)
Publisher Funpec-editora
Extent UNSP gmr.15017055
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000373880200012

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