Mapping and validation of xanthomonas citri subsp citri genes regulated by putative plant-inducible promoter box (pip-box)

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dc.contributor.author Carvalho, F. M. S.
dc.contributor.author Oliveira, J. C. F. [UNIFESP]
dc.contributor.author Laia, M. L.
dc.contributor.author Jacob, T. R.
dc.contributor.author Ferreira, R. M.
dc.contributor.author Ferro, M. I. T.
dc.contributor.author Tezza, R. I. D.
dc.contributor.author Zingaretti, S. M.
dc.contributor.author Silva, C. F.
dc.contributor.author Ferro, J. A.
dc.date.accessioned 2019-01-21T10:29:47Z
dc.date.available 2019-01-21T10:29:47Z
dc.date.issued 2016
dc.identifier http://dx.doi.org/10.4238/gmr.15028428
dc.identifier.citation Genetics And Molecular Research. Ribeirao preto, v. 15, n. 2, p. UNSP 15028428, 2016.
dc.identifier.issn 1676-5680
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/49388
dc.description.abstract Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp citri (Xac), is a major disease affecting citriculture worldwide, because of the susceptibility of the host and the lack of efficient control methods. Previous studies have reported that some genes of phytopathogenic bacteria possess a consensus nucleotide sequence (TTCGC...N15...TTCGC) designated the "plant-inducible-promoter box" (PIP box) located in the promoter region, which is responsible for activating the expression of pathogenicity and virulence factors when the pathogen is in contact with the host plant. In this study, we mapped and investigated the expression of 104 Xac genes associated with the PIP box sequences using a macroarray analysis. Xac gene expression was observed during in vitro (Xac grown for 12 or 20 h in XAM1 induction medium) or in vivo (bacteria grown in orange leaves for 3 to 5 days) infection conditions. Xac grown in non-induction NB liquid medium was used as the control. cDNA was isolated from bacteria grown under the different conditions and hybridized to the macroarray, and 32 genes differentially expressed during the infection period (in vitro or in vivo induction) were identified. The macroarray results were validated for some of the genes through semi-quantitative RT-PCR, and the functionality of the PIP box-containing promoter was demonstrated by activating beta-glucuronidase reporter gene activity by the PIP box-containing promoter region during Xac-citrus host interaction. en
dc.description.sponsorship Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorship Coodernacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.format.extent UNSP 15028428
dc.language.iso eng
dc.publisher Dove Medical Press Ltd
dc.relation.ispartof Genetics And Molecular Research
dc.rights Acesso aberto
dc.subject Pip-Box Promoter en
dc.subject Gene Reporter Beta-Glucuronidase en
dc.subject Citrus Canker en
dc.subject Xanthomonas Citri en
dc.subject Xami Induction MediumCampestris Pv Vesicatoria en
dc.subject Iii Secretion System en
dc.subject Ralstonia-Solanacearum en
dc.subject Mycobacterium-Tuberculosis en
dc.subject Expression en
dc.subject Sequence en
dc.subject Identification en
dc.subject Virulence en
dc.subject Oryzae en
dc.subject Activation en
dc.title Mapping and validation of xanthomonas citri subsp citri genes regulated by putative plant-inducible promoter box (pip-box) en
dc.type Artigo
dc.description.affiliation Laboratório de Bioquímica e Biologia Molecular, Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista,Jaboticabal, SP, Brasil
dc.description.affiliation Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil
dc.description.affiliation Departamento de Ciências Biológicas, Universidade Federal de São Paulo, Diadema, SP, Brasil
dc.description.affiliation Departamento de Engenharia Florestal, Faculdade de Ciências Agrárias, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, MG, Brasil
dc.description.affiliation Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, SP, Brasil
dc.description.affiliation Centro de Recursos Biológicos e Biologia Genômica, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brasil
dc.description.affiliationUnifesp Departamento de Ciências Biológicas, Universidade Federal de São Paulo, Diadema, SP, Brasil
dc.identifier.doi 10.4238/gmr.15028428
dc.description.source Web of Science
dc.identifier.wos WOS:000384881000120



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