Concentration of glycosaminoglycan in ovariectomized mice uterus after treatment with ovarian steroids

Concentration of glycosaminoglycan in ovariectomized mice uterus after treatment with ovarian steroids

Author Maioral, Gabriela C. C. C. Autor UNIFESP Google Scholar
Gomes, Regina Celia T. Autor UNIFESP Google Scholar
Verna, Carina Autor UNIFESP Google Scholar
Simoes, Manuel de J. Autor UNIFESP Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Simoes, Ricardo S. Google Scholar
Baracat, Edmund C. Google Scholar
Soares-, Jose Maria, Jr. Google Scholar
Abstract The aim of this study was to evaluate the amount of non-and sulfated glycosaminoglycans in the ovariectomized mice uterus, after treatment with ovarian steroids. For this purpose, 50 adult female mice were divided into five groups with 10 animals/each: control group: CG (ovary intact), and ovariectomized groups: OG (vehicle), EG (estradiol), PG (progesterone) and EPG (estradiol combined to progesterone). The treatments started 30 days after ovariectomy. All the animals were treated for 50 consecutive days. These hormones were administered in a sterile oily solution via gavage. Twenty-four hours after the last treatment, all animals were euthanized, removing the uterine horn for biochemical analyses. To quantify, the hyaluronic acid (HA) used ELISA-like fluorometric assay, and the sulfated glycosaminoglycans (GAGs) used agarose gel electrophoresis. The amount of HA was significantly higher in the group treated with progesterone (PG) compared to the others groups (p < 0.05), and in the group treated with estradiol (EG), the amount of chondroitin/dermatan sulfate was significantly higher compared to the others groups (p < 0.05), and in the group treated with progesterone (PG), the amount of heparan sulfate was significantly lower compared to the others groups, except to control group (p < 0.05). Our results showed that the estroprogestative therapy after long time (50 days) profoundly affected the amount of glycosaminoglycans in uterine. These changes may be indicative of uterine pathology such as the development of tumor.
Keywords Hyaluronic Acid
Ovarian Steroids
Sulfated GlycosaminoglycansExtracellular-Matrix
Human Endometrium
Heparan-Sulfate
Mouse
Proteoglycans
Progesterone
Hyaluronan
Estrogen
Expression
Estradiol
Language English
Sponsor Foundation for Research Support of Sao Paulo (FAPESP) [2009/52758-9]
Grant number FAPESP: 2009/52758-9
Date 2016
Published in Gynecological Endocrinology. Abingdon, v. 32, n. 8, p. 617-621, 2016.
ISSN 0951-3590 (Sherpa/Romeo, impact factor)
Publisher Hindawi Publishing Corp
Extent 617-621
Origin https://doi.org/10.3109/09513590.2016.1147027
Access rights Closed access
Type Article
Web of Science ID WOS:000387480900008
URI http://repositorio.unifesp.br/handle/11600/49361

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