Effects of acute aerobic exercise on leukocyte inflammatory gene expression in systemic lupus erythematosus

Effects of acute aerobic exercise on leukocyte inflammatory gene expression in systemic lupus erythematosus

Author Perandini, L. A. Google Scholar
Sales-de-Oliveira, Diego Google Scholar
Almeida, D. C. Autor UNIFESP Google Scholar
Azevedo, Hatylas Google Scholar
Moreira-Filho, C. A. Google Scholar
Cenedeze, Marco Antonio Autor UNIFESP Google Scholar
Benatti, F. B. Google Scholar
Lima, F. R. Google Scholar
Borba, Eduardo Google Scholar
Bonfa, E. Google Scholar
Sa-Pinto, A. L. Google Scholar
Roschel, H. Google Scholar
Camara, N. O. Google Scholar
Gualano, B. Google Scholar
Abstract Systemic lupus erythematosus (SLE) is an autoimmune disease with a persistent systemic inflammation. Exercise-induced inflammatory response in SLE remains to be fully elucidated. The aim of this study was to assess the effects of acute exercise on leukocyte gene expression in active (SLEACTIVE) and inactive SLE (SLEINACTIVE) patients and healthy controls (HC). Methods: All subjects (n = 4 per group) performed a 30-min single bout of acute aerobic exercise (similar to 70% of VO2 peak) on a treadmill, and blood samples were collected for RNA extraction from circulating leukocyte at baseline, at the end of exercise, and after three hours of recovery. The expression of a panel of immune-related genes was evaluated by a quantitative PCR array assay. Moreover, network-based analyses were performed to interpret transcriptional changes occurring after the exercise challenge. Results: In all groups, a single bout of acute exercise led to the down-regulation of the gene expression of innate and adaptive immunity at the end of exercise (e.g., TLR3, IFNG, GATA3, FOXP3, STAT4) with a subsequent up-regulation occurring upon recovery. Exercise regulated the expression of inflammatory genes in the blood leukocytes of the SLE patients and HC, although the SLE groups exhibited fewer modulated genes and less densely connected networks (number of nodes: 29, 40 and 58

number of edges: 29, 60 and 195

network density: 0.07, 0.08 and 0.12, for SLEACTIVE, SLEINACTIVE and HC, respectively). Conclusion: The leukocytes from the SLE patients, irrespective of disease activity, showed a down-regulated inflammatory gene expression immediately after acute aerobic exercise, followed by an up-regulation at recovery. Furthermore, less organized gene networks were observed in the SLE patients, suggesting that they may be deficient in triggering a normal exercise-induced immune transcriptional response.
Keywords Physical Activity
Inflammation
Autoimmunity
Gene Array
Exercise Immunology
Network Analysis
HubsBlood Mononuclear-Cells
Autoimmune Rheumatic-Diseases
Immune-Response
Tnf-Alpha
Accelerated Atherosclerosis
Microrna Expression
Multiple-Sclerosis
Physical-Activity
Heart-Failure
Messenger-Rna
Language English
Sponsor Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Date 2016
Published in Exercise Immunology Review. Greven, v. 22, p. 64-80, 2016.
ISSN 1077-5552 (Sherpa/Romeo, impact factor)
Publisher Centro Federal Educacao Tecnologica Rio Grande Norte
Extent 64-80
Origin http://eir-isei.de/2016/eir-2016-064-article.pdf
Access rights Open access Open Access
Type Revisão
Web of Science ID WOS:000387132000005
URI http://repositorio.unifesp.br/handle/11600/49360

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