Influence of the expression of inflammatory markers on kidney after fetal programming in an experimental model of renal failure

Influence of the expression of inflammatory markers on kidney after fetal programming in an experimental model of renal failure

Author Pereira Junior, Carlos Donizete Google Scholar
de Oliveira Guimaraes, Camila Souza Google Scholar
Souza da Silva, Aline Cristina Google Scholar
Aquiles Rodrigues, Aldo Rogelis Google Scholar
da Gloria, Maria Aparecida Autor UNIFESP Google Scholar
Antunes Teixeira, Vicente de Paula Google Scholar
Saraiva Camara, Niels Olsen Autor UNIFESP Google Scholar
Rocha, Lenaldo Branco Google Scholar
dos Reis, Marlene Antonia Google Scholar
Machado, Juliana Reis Google Scholar
Rocha, Laura Penna Google Scholar
Helmo, Fernanda Rodrigues Google Scholar
Miranda Correa, Rosana Rosa Google Scholar
Abstract Objective. To evaluate the expression of inflammatory markers in experimental renal failure after fetal programming. Methods. The offspring aged two and fivemonths were divided into four groups: CC (control dams, control offspring)

DC (diabetic dams, control offspring)

CFA (control dams, folic acid offspring, 250 mg/Kg)

and DFA (diabetic dams, folic acid offspring). Gene expression of inflammatory markersMCP-1, IL-1, NOS3, TGF-beta, TNF-alpha, and VEGF was evaluated by RT-PCR. Results. MCP-1 was increased in the CFA and DFA groups at two and fivemonths of age, as well as in DC5 when compared to CC5. There was a higher expression of IL-1 in the CFA2, DFA2, and DC2 groups. There was a decrease in NOS3 and an increase in TNF-alpha in DFA5 in relation to CFA5. The gene expression of TGF-beta increased in cases that had received folic acid at two and five months, and VEGF decreased in the CFA5 and DFA5 groups. DC5 showed increased VEGF expression in comparison with CC5. Conclusions. Gestational diabetes mellitus and folic acid both change the expression of inflammatory markers, thus demonstrating that the exposure to harmful agents in adulthood has a more severe impact in cases which underwent fetal reprogramming.
Keywords Endothelial Growth-Factor
Diabetic-Nephropathy
Holocaust Survivors
Insulin-Resistance
E-Selectin
Tnf-Alpha
Disease
Genotoxicity
Pathogenesis
Pregnancy
Language English
Sponsor Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)
Fundacao de Ensino e Pesquisa de Uberaba (FUNEPU)
Date 2016
Published in Journal Of Immunology Research. New york, 2016.
ISSN 2314-8861 (Sherpa/Romeo, impact factor)
Publisher Czech Acad Sci, Inst Entomology
Origin http://dx.doi.org/10.1155/2016/9151607
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000390014700001
URI http://repositorio.unifesp.br/handle/11600/49330

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