Effects of early and late treatment with soy isoflavones in the mammary gland of ovariectomized rats

Effects of early and late treatment with soy isoflavones in the mammary gland of ovariectomized rats

Author Santos, Miriam Aparecida Autor UNIFESP Google Scholar
Florencio-Silva, Rinaldo Autor UNIFESP Google Scholar
Teixeira, Cristiane de Paula Autor UNIFESP Google Scholar
Sasso, Gisela Rodrigues da Silva Autor UNIFESP Google Scholar
Marinho, Darci Souza Autor UNIFESP Google Scholar
Simões, Ricardo Santos Google Scholar
Simões, Manuel de Jesus Autor UNIFESP Google Scholar
Carbonel, Adriana Aparecida Ferraz Autor UNIFESP Google Scholar
Abstract Introduction Soy isoflavones have been shown to be an alternative to hormone therapy at menopause, without causing side-effects such as breast cancer. However, the effects of early and late treatment with isoflavones on the mammary gland remain controversial.Objective To investigate the effects of early and late treatment with soy isoflavones on the mammary gland of ovariectomized rats.Methods Thirty 3-month-old rats were ovariectomized and divided equally into groups: Control, treated with vehicle solution

or with 150mg/kg/body weight of isoflavones by gavage

or subcutaneously treated with 10g/kg/body weight with 17-estradiol. Treatments started 3 days (early treatment) or 30 days (late treatment) after ovariectomy and lasted for 30 consecutive days. Thereafter, the animals were euthanized and the mammary glands were removed and processed for paraffin embedding. Sections were stained with hematoxylin and eosin for histomorphometry or subjected to immunohistochemical detection of Ki-67 and VEGF-A.Results The ductal, lobular and total epithelial fractions were similar between controls and the early/late isoflavone groups, but they were significantly higher in the groups treated with estradiol. In both epithelial and stromal regions, the immunoreactivity of VEGF-A and the percentage of Ki-67-positive cells were significantly higher in the groups treated with estradiol, while they were similar in the early/late isoflavone groups and control groups.Conclusion Our results indicate that early and late treatment with soy isoflavones at the dose of 150mg/kg/body weight does not show proliferative and angiogenic effects on the mammary gland of ovariectomized rats.
Keywords Isoflavones
Mammary Gland
Menopause
Rat
EstrogenBreast-Cancer Cells
Anti-Angiogenic Therapy
Mouse Model
Estradiol Deprivation
Estrogen-Sensitivity
Hormone-Therapy
Bone Tissue
Phytoestrogens
Growth
Risk
Language English
Sponsor CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
Date 2016
Published in Climacteric. Abingdon, v. 19, n. 1, p. 77-84, 2016.
ISSN 1369-7137 (Sherpa/Romeo, impact factor)
Publisher Cuba Editora
Extent 77-84
Origin https://doi.org/10.3109/13697137.2015.1094783
Access rights Closed access
Type Article
Web of Science ID WOS:000367611600012
URI http://repositorio.unifesp.br/handle/11600/49203

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