Neurogenesis induced by seizures in the dentate gyrus is not related to mossy fiber sprouting but is age dependent in developing rats

Neurogenesis induced by seizures in the dentate gyrus is not related to mossy fiber sprouting but is age dependent in developing rats

Alternative title A neurogênese induzida por crises no giro denteado não está relacionada ao brotamento de fibras musgosas, mas é dependente da idade, em ratos durante o desenvolvimento
Author Sanabria, Yaima Del Carmen Garrido Autor UNIFESP Google Scholar
Argañaraz, Gustavo Adolfo Autor UNIFESP Google Scholar
Lima, Eliângela de Autor UNIFESP Google Scholar
Priel, Margareth Rose Autor UNIFESP Google Scholar
Trindade, Edvaldo da Silva Autor UNIFESP Google Scholar
Loeb, Luana Mazzacoratti Autor UNIFESP Google Scholar
Scorza, Fulvio Alexandre Autor UNIFESP Google Scholar
Cavalheiro, Esper Abrão Autor UNIFESP Google Scholar
Amado, Débora Autor UNIFESP Google Scholar
Naffah-Mazzacoratti, Maria da Graca Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Neurogenesis in the dentate gyrus (DG) has attracted attention since abnormal supragranular mossy fiber sprouting occurs in the same region, in temporal lobe epilepsy. Thus, we submitted developing rats to pilocarpine-induced status epilepticus (SE) to study the relationship between neurogenesis and mossy fiber sprouting. Groups were submitted to SE at: I-P9, II-P7, P8 and P9, III-P17 e IV-P21. Neurogenesis was quantified using BrdU protocol and confirmed through double staining, using neuronal pentraxin. Other animals were monitored by video system until P120 and their brain was studied (Timm and Nissl staining). The neurogenesis at P17 (p=0.007) and P21 (p=0.006) were increased. However, only P21 group showed recurrent seizures and the mossy fiber sprouting in the same region, during adult life, while P17 did not. Thus, our results suggest that neurogenesis is not related to mossy fiber sprouting neither to recurrent spontaneous seizures in pilocarpine model.

A neurogênese no giro dentado tem atraído atenção já que ela ocorre na mesma região do hipocampo que o brotamento das fibras musgosas, na epilepsia do lobo temporal. Assim, submetemos ratos em desenvolvimento ao status epilepticus induzido (SE) por pilocarpine. Grupos foram submetidos em I-P9, II-P7, P8, P9; III-P17 e IV-P21. A neurogênese foi observada usando o protocolo do BrdU e confirmada por dupla marcação com pentraxina neuronal. Outros animais foram monitorados até P120 e seus cérebros analisados (Nissl e Timm). A neurogênese nos grupos P17 (p=0,007) e P21 (p=0,006) aumentaram. Entretanto, o P21 apresentou crises espontâneas e brotamento de fibras musgosas, na mesma região onde ocorreu a neurogênese, enquanto o grupo P17 apresentou somente aumento na neurogênese. Assim, nossos resultados sugerem que o fenômeno da neurogênese não está relacionado com o brotamento de fibras musgosas nem com o aparecimento de crises espontâneas e recorrentes no modelo da pilocarpina.
Keywords neurogenesis
epilepsy
development
pilocarpine
BrdU
immunohistochemistry
epilepsia
desenvolvimento
neurogênese
pilocarpina
BrdU
Language English
Date 2008-12-01
Published in Arquivos de Neuro-Psiquiatria. Academia Brasileira de Neurologia - ABNEURO, v. 66, n. 4, p. 853-860, 2008.
ISSN 0004-282X (Sherpa/Romeo, impact factor)
Publisher Academia Brasileira de Neurologia - ABNEURO
Extent 853-860
Origin http://dx.doi.org/10.1590/S0004-282X2008000600015
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000262070600015
SciELO ID S0004-282X2008000600015 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/4720

Show full item record




File

Name: S0004-282X2008000600015.pdf
Size: 2.407Mb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account