P2X(7) receptor and klotho expressions in diabetic nephropathy progression

P2X(7) receptor and klotho expressions in diabetic nephropathy progression

Author Rodrigues, A. M. Autor UNIFESP Google Scholar
Serralha, R. S. Autor UNIFESP Google Scholar
Farias, C. Autor UNIFESP Google Scholar
Punaro, Giovana Rita Autor UNIFESP Google Scholar
Fernandes, M. J. S. Autor UNIFESP Google Scholar
Higa, Elisa Mieko Suemitsu Autor UNIFESP Google Scholar
Abstract Diabetes mellitus is characterized by increased levels of reactive oxygen species (ROS), leading to high levels of adenosine triphosphate (ATP) and the activation of purinergic receptors (P2X(7)), which results in cell death. Klotho was recently described as a modulator of oxidative stress and as having anti-apoptotic properties, among others. However, the roles of P2X(7) and klotho in the progression of diabetic nephropathy are still unclear. In this context, the aim of the present study was to characterize P2X(7) and klotho in several stages of diabetes in rats. Diabetes was induced in Wistar rats by streptozotocin, while the control group rats received the drug vehicle. From the 1st to 8th weeks after the diabetes induction, the animals were placed in metabolic cages on the 1st day of each week for 24 h to analyze metabolic parameters and for the urine collection. Then, blood samples and the kidneys were collected for biochemical analysis, including Western blotting and qPCR for P2X(7) and klotho. Diabetic rats presented a progressive loss of renal function, with reduced nitric oxide and increased lipid peroxidation. The P2X(7) and klotho expressions were similar up to the 4th week

then, P2X(7) expression increased in diabetes mellitus (DM), but klotho expression presented an opposite behavior, until the 8th week. Our data show an inverse correlation between P2X(7) and klotho expressions through the development of DM, which suggests that the management of these molecules could be useful for controlling the progression of this disease and diabetic nephropathy.
Keywords Diabetes mellitus
Oxidative stress
Purinergic receptor
Language English
Sponsor Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Fundacao de Apoio a Pesquisa da UNIFESP (FAP)
Date 2018
Published in Purinergic Signalling. Dordrecht, v. 14, n. 2, p. 167-176, 2018.
ISSN 1573-9538 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 167-176
Origin http://dx.doi.org/10.1007/s11302-018-9602-y
Access rights Closed access
Type Article
Web of Science ID WOS:000431794000006
URI http://repositorio.unifesp.br/handle/11600/46014

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