Optical properties and antimicrobial effects of silver nanoparticles synthesized by femtosecond laser photoreduction

Optical properties and antimicrobial effects of silver nanoparticles synthesized by femtosecond laser photoreduction

Author Courrol, Daniella dos Santos Google Scholar
Lopes, Carla Regina Borges Autor UNIFESP Google Scholar
Cordeiro, Thiago da Silva Google Scholar
Franzolin, Marcia Regina Google Scholar
Vieira Junior, Nilson Dias Google Scholar
Sarnad, Ricardo Elgul Google Scholar
Courrol, Lilia Coronato Autor UNIFESP Google Scholar
Abstract Silver nanoparticles exhibit a powerful antimicrobial action showing a pronounced potential to be widely used against drug resistance bacteria. The present work describes the optical properties and antimicrobial effect of silver nanoparticles produced by femtosecond laser photoreduction of AgNO3 in the presence of tryptophan water solution. The advantages of this method are the absence of hazardous chemical reducing agents in the solution, and the versatile dimensional control achieved. The synthesized silver nanoparticles were characterized by absorption and fluorescence spectroscopy and their antibacterial activity were determined by monitoring the cell viability of Escherichia coli. The effects of the silver nanoparticles concentration and laser parameters (exposure time and pulse energy), on the formation of the nanoparticles, and its influence on the bacteria growth inhibition were studied. The prepared silver nanoparticles exhibited suitable antimicrobial properties. The results demonstrated that the nanoparticles concentration plays an important role in their bactericidal efficacy. The increase in the laser energy caused an increase in E. coli growth inhibition. Irradiations with energies around 300 mu j for 60 min presented high antimicrobial activity due to the presence of kynurenine, sub product of tryptophan photolysis. The first-time formation mechanism of tryptophan silver nanoparticles in high optical intensities was also discussed. (C) 2018 Elsevier Ltd. All rights reserved.
Keywords Silver nanoparticles
Microorganisms
Laser
Femtosecond
Tryptophan
Language English
Sponsor National Council for Scientific and Technological Development (CNPq)
Sao Paulo State Research Support Foundation
PAP
Grant number FAPESP: 2014/06960-9
Date 2018
Published in Optics And Laser Technology. Oxford, v. 103, p. 233-238, 2018.
ISSN 0030-3992 (Sherpa/Romeo, impact factor)
Publisher Elsevier Sci Ltd
Extent 233-238
Origin http://dx.doi.org/10.1016/j.optlastec.2018.01.044
Access rights Closed access
Type Article
Web of Science ID WOS:000427339000031
URI http://repositorio.unifesp.br/handle/11600/45972

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