Tap Water has No Influence on Microbial Colonization of Skin Wounds in Rats

Tap Water has No Influence on Microbial Colonization of Skin Wounds in Rats

Author Resende, Margarida Google Scholar
Hochman, Bernardo Autor UNIFESP Google Scholar
Gragnani, Alfredo Autor UNIFESP Google Scholar
Viega, Daniela Google Scholar
Damasceno, Carlos Google Scholar
Juliano, Yara Autor UNIFESP Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Vale Sapucai
Abstract The aim of this study was to investigate the influence of tap water on microbial colonization of skin wounds in rats. Methods. A circular wound 2.4 cm in diameter was created on the back of 40 Wistar rats, which were randomly assigned to the control (n = 20) or study (n 20) group. The wounds were cleansed daily using a sterile 0.9% sodium chloride solution (control group) or tap water (study group) for 6 days, following a standardized protocol. Sequential samples were collected for microbiological analysis every other day. Results. Microbial growth occurred in 49% of the samples from the control group and in 47% of the samples from the study group (P = 0.39). There was no significant difference in microbial growth between groups for the different culture media: thioglycolate (P = 0.20), mannitol salt agar (P = 0.53), blood agar (P = 0.61), eosin methylene blue agar (P = 0.51), and Sabouraud agar (P = 0.34). The following microorganisms were identified: Bacillus subtilis, Staphylococcus sp, Aureobasidium sp, Penicillium sp, and Cladosporium sp. Conclusion. The use of tap water for cleansing skin wounds in rats had no effect on microbial colonization compared with the use of a sterile saline solution.
Language English
Date 2012-09-01
Published in Wounds-a Compendium Of Clinical Research And Practice. Malvern: H M P Communications, v. 24, n. 9, p. 275-282, 2012.
ISSN 1044-7946 (Sherpa/Romeo, impact factor)
Publisher H M P Communications
Extent 275-282
Origin http://www.woundsresearch.com/article/tap-water-has-no-influence-microbial-colonization-skin-wounds-rats
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000308778300007
URI http://repositorio.unifesp.br/11600/45757

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