A novel rat contact lens model for Fusarium keratitis

A novel rat contact lens model for Fusarium keratitis

Author Abou Shousha, Mohamed Google Scholar
Santos, Andrea Rachelle C. Google Scholar
Oechsler, Rafael Allan Autor UNIFESP Google Scholar
Iovieno, Alfonso Google Scholar
Maestre-Mesa, Jorge Google Scholar
Ruggeri, Marco Google Scholar
Echegaray, Jose J. Google Scholar
Dubovy, Sander R. Google Scholar
Perez, Victor L. Google Scholar
Miller, Darlene Google Scholar
Alfonso, Eduardo C. Google Scholar
Bajenaru, M. Livia Google Scholar
Institution Univ Miami
St Louis Univ
Universidade Federal de São Paulo (UNIFESP)
Univ Campus Biomed
Abstract Purpose: The aim of this study was to develop and characterize a new contact lens-associated fungal keratitis rat model and to assess the ability of non-invasive spectral-domain optical coherence tomography (SD-OCT) to detect pathological changes in vivo in fungal keratitis.Methods: We used SD-OCT to image and measure the cornea of Sprague Dawley rats. Fusarium infection was initiated in the rat eye by fitting Fusarium solani-soaked contact lenses on the experimental eye, while the control animals received contact lenses soaked in sterile saline. The fungal infection was monitored with periodic slit-lamp examination and in vivo SD-OCT imaging of the rat eye, and confirmed by histology, counting of viable fungi in the infected rat cornea, and PCR with specific primers for Fusarium sp.Results: We imaged and measured the rat cornea with SD-OCT. Custom-made contact lenses were developed based on the OCT measurements. Incubation of contact lenses in a F. solani suspension resulted in biofilm formation. We induced contact lens-associated Fusarium keratitis by fitting the rat eyes for 4 h with the Fusarium-contaminated contact lenses. The SD-OCT images of the cornea correlated well with the slit-lamp and histopathological results and clearly defined clinical signs of infection, namely, increased corneal thickening, loss of epithelial continuity, hyper-reflective areas representing infiltrates, and endothelial plaques characteristic of fungal infection. Moreover, in three cases, SD-OCT detected the infection without any clear findings on slit-lamp examination. Infection was confirmed with histological fungal staining, PCR, and microbiological culture positivity.Conclusions: We developed a highly reproducible rat contact lens model and successfully induced contact lens-associated Fusarium keratitis in this model. The clinical presentation of contact lens-associated Fusarium keratitis in the rat model is similar to the human condition. SD-OCT is a valuable tool that non-invasively revealed characteristic signs of the fungal infection and could provide sensitive, objective monitoring in fungal keratitis.
Language English
Sponsor Allergan Horizon Fellowship Grant
University of Miami Scientific Awards Committee Pilot Grant
National Institutes of Health
Research to Prevent Blindness
Grant number National Institutes of Health: NIHP301430
Date 2013-12-27
Published in Molecular Vision. Atlanta: Molecular Vision, v. 19, p. 2596-2605, 2013.
ISSN 1090-0535 (Sherpa/Romeo, impact factor)
Publisher Molecular Vision
Extent 2596-2605
Origin http://www.molvis.org/molvis/v19/2596/
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000331821400002
URI http://repositorio.unifesp.br/11600/44938

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