Wade histoid Leprosy: histological and immunohistochemical analysis

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dc.contributor.author Costa, Daniela Aidar Monteiro da [UNIFESP]
dc.contributor.author Enokihara, Mílvia Maria Simões e Silva [UNIFESP]
dc.contributor.author Nonogaki, Suely
dc.contributor.author Maeda, Solange Miki [UNIFESP]
dc.contributor.author Porro, Adriana Maria [UNIFESP]
dc.contributor.author Tomimori, Jane [UNIFESP]
dc.date.accessioned 2018-06-18T10:54:49Z
dc.date.available 2018-06-18T10:54:49Z
dc.date.issued 2013-09-01
dc.identifier http://www.lepra.org.uk/platforms/lepra/files/lr/Sept13/1839.pdf
dc.identifier.citation Leprosy Review. Colchester: Lepra, v. 84, n. 3, p. 176-185, 2013.
dc.identifier.issn 0305-7518
dc.identifier.uri http://repositorio.unifesp.br/11600/44853
dc.description.abstract Histoid leprosy is a rare multibacillary form that presents with disseminated papule-nodular cutaneous lesions. To study the inflammatory infiltrate of the histoid form and to compare it with other lepromatous forms, we performed histological and immunohistochemical analysis on skin biopsies. Fifteen patients were included for histopathological analysis (10 histoid and five lepromatous) via the haematoxylin-eosin and Ziehl-Neelsen-Faraco stains. Thus, immunohistochemical techniques using immunoperoxidase assay were performed for: anti-BCG, anti-M. leprae, anti-CD8, anti-CD3, anti-CD20, anti-S100, anti-CD1a, anti-CD68 and antivimentin. Spindle cells were present in all histoid patients. A pseudocapsule was observed in half of both studied forms. A comparison using the Ziehl-Neelsen-Faraco stain to evaluate anti-BCG and anti-M.leprae showed no major differences. The CD3+ cells were more pronounced in the histoid form than the lepromatous form. There was greater immunoreactivity toward CD8+ cells in the histoid form, as well as the CD20+ cell count. A similar count of S100+ cells in the epidermis of both leprosy forms was observed. There was a slight increase of dendritic cells in the histoid patients in the superficial and deep dermis. For CD1a marker, we observed expression in the epidermis and superficial dennis in both forms. A diffuse and intense infiltrate of CD68+ cells was also observed in the histoid and lepromatous forms. The high positivity for vimentin did not allow for a positive cell count. We concluded that the activation of both the cellular and humoral response is more pronounced in the histoid form because the T and B cells showed greater infiltration than those in the lepromatous form. The activation of dendritic and Langerhans cells is similar in both forms. The spindle cells likely belong to the macrophage population, thus maintaining phagocytic ability. The quantities of pseudocapsules and bacilli are similar and cannot serve as criteria for diagnosis. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Brazilian Ministry of Science and Technology
dc.format.extent 176-185
dc.language.iso eng
dc.publisher Lepra
dc.relation.ispartof Leprosy Review
dc.rights Acesso aberto
dc.title Wade histoid Leprosy: histological and immunohistochemical analysis en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Adolfo Lutz Inst
dc.description.affiliation Escola Paulista Med UNIFESP, BR-04038001 Sao Paulo, Brazil
dc.description.affiliation Escola Paulista Med UNIFESP, Dept Pathol, BR-04038001 Sao Paulo, Brazil
dc.description.affiliation Adolfo Lutz Inst, Pathol Ctr, Sao Paulo, Brazil
dc.description.affiliation Escola Paulista Med UNIFESP, Dept Dermatol, BR-04038001 Sao Paulo, Brazil
dc.description.affiliationUnifesp Escola Paulista Med UNIFESP, BR-04038001 Sao Paulo, Brazil
dc.description.affiliationUnifesp Escola Paulista Med UNIFESP, Dept Pathol, BR-04038001 Sao Paulo, Brazil
dc.description.affiliationUnifesp Escola Paulista Med UNIFESP, Dept Dermatol, BR-04038001 Sao Paulo, Brazil
dc.description.source Web of Science
dc.identifier.wos WOS:000328999900002



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