Neutral endopeptidase expression in mesangial cells

Neutral endopeptidase expression in mesangial cells

Author Ebihara, Fabiana Autor UNIFESP Google Scholar
Di Marco, Giovana Seno Autor UNIFESP Google Scholar
Juliano, Maria Aparecida Autor UNIFESP Google Scholar
Casarini, Dulce Elena Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract In the kidney, neutral endopeptidase (NEP) is implicated in the metabolism of several peptides involved in blood pressure and sodium homeostasis control, such as the atrial natriuretic peptide, bradykinin and angiotensin I. Due to its physiological importance in the modulation of pressor responses, the presence of NEP in mouse mesangial cells has been investigated, since these cells control glomerular function and are able to synthesise components of the renin-angiotensin system. A NEP-like activity (NEP-like) that cleaves the fluorogenic substrates Abz-BKQ-EDDnp and AbZ-DRRL-EDDnp was purified from mesangial cell lysate by ion-exchange, followed by gel filtration chromatography. The enzyme was able to hydrolyse bradykinin at the G(4)-F-5 peptide bond and was inhibited by thiorphan. A pH study established that enzyme activity was maximal at pH 7.5 and the determined K-m was 4.86 muM using AbZ-DRRL-EDDnp as substrate. NEP-like was recognised by monoclonal anti-NEP and had a molecular mass of 95 kDa. The purified enzyme was sequenced and showed similarity with human, rat, mouse and rabbit NEPs. We isolated, for the first time, NEP-like from mesangial cells. This enzyme could have an important role in the renal physiology by its action upon different peptides that are able to alter renal haemodynamics.
Keywords neutral
mesangial cells
Language English
Date 2003-12-01
Published in Journal Of The Renin-angiotensin-aldosterone System. Birmingham: J R A A S Ltd, v. 4, n. 4, p. 228-233, 2003.
ISSN 1470-3203 (Sherpa/Romeo, impact factor)
Publisher J R A A S Ltd
Extent 942-943
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000187427900005

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