Histochemical and ultrastructural study of the extracellular matrix fibers in patellar tendon donor site scars and normal controls

Histochemical and ultrastructural study of the extracellular matrix fibers in patellar tendon donor site scars and normal controls

Author Battlehner, Cláudia Naves Google Scholar
Carneiro Filho, Mario Autor UNIFESP Google Scholar
Ferreira Junior, Jorge Mario da Costa Google Scholar
Saldiva, Paulo Hilário Nascimento Autor UNIFESP Google Scholar
Montes, Gregorio Santiago Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
BUTANTAN INST
Abstract Lesions of the anterior cruciate ligament are extremely common and frequently demand surgical treatment in order to avoid disabling sequels. The use of a central one-third of the patellar tendon as an autograft for surgical reconstruction of a damaged cruciate ligament is common. Although several investigations in human and animal models have demonstrated long-term graft viability, there have been cases of loosening and rupture of the graft. In these occasions, a new substitute for the torn structure must be found. Owing to its inherent accessibility, the patellar tendon has been elected one of the choices of donor tissue. In order to evaluate the characteristics of the remaining scar, we performed a histochemical and ultrastructural study using biopsy material obtained from the central one-third of the donor tendon of 8 patients. This material was analyzed by comparing the ultrastructural picture with the results obtained using the specific method for collagen-containing fibers (Picrosirius-polarization) by light microscopy; four normal patellar tendons were used as controls. Despite the resemblance with the normal tissue, our results show that Phr healed tissue does nor restore the tendon ad integrum, neither at the light microscopic nor at the electron microscopic levels. Structural differences can be responsible for biomechanical alterations. Impaired biomechanical properties can, at least partly, explain some of the clinical complications observed in patients submitted to this surgical technique. However, without performing biomechanical studies in this kind of tendons, we are neither allowed to encourage nor to reject the use of scars as donor tissue for a second surgery.
Keywords patellar tendon scars
extracellular matrix fibers
morphometry
histochemistry
ultrastructure
Language English
Date 1996-04-01
Published in Journal Of Submicroscopic Cytology And Pathology. Bologna: Editrice Compositori Bologna, v. 28, n. 2, p. 175-186, 1996.
ISSN 1122-9497 (Sherpa/Romeo, impact factor)
Publisher Editrice Compositori Bologna
Extent 175-186
Access rights Closed access
Type Article
Web of Science ID WOS:A1996UJ39300005
URI http://repositorio.unifesp.br/11600/44711

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account