Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii

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dc.contributor.author Toledo, Marcos S. [UNIFESP]
dc.contributor.author Levery, Steven B.
dc.contributor.author Straus, Anita H. [UNIFESP]
dc.contributor.author Takahashi, Helio K. [UNIFESP]
dc.date.accessioned 2018-06-15T18:07:20Z
dc.date.available 2018-06-15T18:07:20Z
dc.date.issued 2000-05-01
dc.identifier http://www.jlr.org/content/41/5/797.abstract
dc.identifier.citation Journal Of Lipid Research. Bethesda: Lipid Research Inc, v. 41, n. 5, p. 797-806, 2000.
dc.identifier.issn 0022-2275
dc.identifier.uri http://repositorio.unifesp.br/11600/44472
dc.description.abstract Major neutral glycosphingolipid components were extracted from Sporothrix schenckii, a dimorphic fungus exhibiting a hyphal saprophytic phase and a yeast parasitic phase responsible for chronic mycotic infections in mammalian hosts. These components, one from the mycelial form and two from the yeast form, were purified and their structures were elucidated by H-1 nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and tandem ESI-MS/MS, All three were characterized as cerebrosides (monohexosylceramides) containing (4E, 8E)-9-methyl-4,8-sphingadienine as the long-chain base attached to N-2'-hydroxyoctadecanoate and N2'-hydroxy-(E)-Delta(3)-octadecenoate as the fatty acyl components. However, while the mycelial form expressed only beta-glucopyranosylceramide, the yeast form expressed both beta-gluco- and beta-galactopyranosylceramides in approximately equal amounts. In addition, while the glucosylceramides of both mycelial and yeast forms had similar proportions of saturated and (E)-Delta(3) unsaturated 2-hydroxy fatty acid, the galactocerebroside of the yeast form had significantly higher levels of (E)Delta(3) unsaturation. The differences in cerebroside hexose structure represent a novel type of glycosphingolipid dimorphism not previously reported in fungi, Possible implications of these findings with respect to regulation of morphological transitions in S, schenckii and other dimorphic fungi are discussed. Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii. en
dc.format.extent 797-806
dc.language.iso eng
dc.publisher Lipid Research Inc
dc.relation.ispartof Journal Of Lipid Research
dc.rights Acesso aberto
dc.subject electrospray ionization en
dc.subject mass spectrometry en
dc.subject ESI-MS en
dc.subject nuclear magnetic resonance spectroscopy en
dc.subject NMR en
dc.subject collision-induced dissociation en
dc.subject tandem mass spectrometry en
dc.subject fungus en
dc.subject yeast en
dc.subject mycosis en
dc.title Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii en
dc.type Artigo
dc.contributor.institution Univ Georgia
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
dc.description.affiliation Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA
dc.description.affiliation Univ Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 Sao Paulo, SP, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 Sao Paulo, SP, Brazil
dc.description.source Web of Science
dc.identifier.wos WOS:000087054200014



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