Nasal provocation test (NPT) with isolated and associated Dermatophagoides pteronyssinus (Dp) and Endotoxin lipopolysaccharide (LPS) in children with allergic rhinitis (AR) and nonallergic controls

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dc.contributor.author Braga, C. R.
dc.contributor.author Rizzo, MCV
dc.contributor.author Naspitz, C. K.
dc.contributor.author Sole, D.
dc.date.accessioned 2018-06-15T18:02:44Z
dc.date.available 2018-06-15T18:02:44Z
dc.date.issued 2004-01-01
dc.identifier http://www.jiaci.org/summary/vol14-issue2-num18
dc.identifier.citation Journal Of Investigational Allergology And Clinical Immunology. Gottingen: Hogrefe & Huber Publishers, v. 14, n. 2, p. 142-148, 2004.
dc.identifier.issn 1018-9068
dc.identifier.uri http://repositorio.unifesp.br/11600/44465
dc.description.abstract Background: Inhalation of endotoxin may enhance inflammatory airway response in sensitized asthmatics persons after allergen(s) inhalation.Objective: To evaluate nasal response to intranasal instillation of Dermatophagoides pteronyssinus (Dp), endotoxin (LPS), and to Dp+LPS in children with perennial allergic rhinitis (PAR).Methods: 10 PAR children (positive skin prick test to Dp) and 10 nonallergic controls (C) undergoing nasal provocation test (NPT), who were quantified by active anterior rhinomanometry and measurement of Total Nasal Resistance (TNR). The NPTs were initially performed with histamine (H; 0.03 to 16.0 mg/mL), and then, at least at weekly intervals, the NPTs were done with Dp (1/100,000 to 1/2.5), LPS (1 to 500 mg/mL) and to Dp+LPS. During NPT with Dp+LPS, Dp concentration was kept constant (1/100,000; 1/10,000; 1/1,000) and was combined with different concentrations of LPS (1, 5, 10 20 mg/mL). The NPT was considered positive when TNR reached twice the basal TNR.Results: H and Dp NPTs were positive in all AR children. In group C, H NPT was positive in 60% and Dp NPT was negative in all children. NPT with LPS was positive only in 30% of the AR children. NPT with Dp+LPS was positive in 90% of the AR patients in Dp concentration of 1/1,000 and in LPS concentrations of 5, 10, and 20 mcg/ mL. This positive association was observed with Dp concentrations lower than those obtained during NPT with Dp in 60% of AR patients. There were no changes in pulmonary function tests in all children after NPT.Conclusions: This study suggests that LPS enhances the effects of allergen challenges on nasal airflow. The daily inhalation of allergens plus endotoxin in AR patients does increase the nasal responsiveness. en
dc.format.extent 142-148
dc.language.iso eng
dc.publisher Hogrefe & Huber Publishers
dc.relation.ispartof Journal Of Investigational Allergology And Clinical Immunology
dc.rights Acesso aberto
dc.subject allergic rhinitis en
dc.subject mites en
dc.subject lipopolysaccharide en
dc.subject LPS en
dc.subject endotoxin en
dc.subject D. pteronyssinus en
dc.title Nasal provocation test (NPT) with isolated and associated Dermatophagoides pteronyssinus (Dp) and Endotoxin lipopolysaccharide (LPS) in children with allergic rhinitis (AR) and nonallergic controls en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Sao Paulo, Dept Pediat, Escola Paulista Med, Div Allergy Clin Immunol & Rheumatol, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Dept Pediat, Escola Paulista Med, Div Allergy Clin Immunol & Rheumatol, Sao Paulo, Brazil
dc.description.source Web of Science
dc.identifier.wos WOS:000222875300008



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